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dc.contributor.authorZhang, Qinghaoen_UK
dc.contributor.authorYou, Cuihongen_UK
dc.contributor.authorWang, Shuqien_UK
dc.contributor.authorDong, Yeweien_UK
dc.contributor.authorMonroig, Oscaren_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorLi, Yuanyouen_UK
dc.description.abstractAs the first marine teleost demonstrated to have the ability to biosynthesize long-chain polyunsaturated fatty acids (LC-PUFA) from C18PUFA precursors, rabbitfishSiganus canaliculatusprovides a good model for studying the regulatory mechanisms of LC-PUFA biosynthesis in teleosts. Here the potential roles of miR-33 in such regulation were investigated. The miR-33 gene was identified within intron 16 of the gene encoding sterol regulatory element-binding protein 1 (Srebp1), an activator of LC-PUFA biosynthesis. Expression of miR-33 in rabbitfish tissues correlated with that ofsrebp1, while its expression in liver was highly responsive to ambient salinities and PUFA components, factors affecting LC-PUFA biosynthesis. Srebp1 activation promoted the expression of Δ4 and Δ6 Δ5 fatty acyl desaturases (Fad), key enzymes for LC-PUFA biosynthesis, accompanied by elevated miR-33 abundance in rabbitfish hepatocytes. miR-33 overexpression induced the expression of the twofad, but suppressed that of insulin-induced gene 1 (insig1), which encodes a repressor blocking Srebp proteolytic activation and has targeting sites of miR-33. These results indicated that miR-33, cooperating with Srebp1, may be involved in regulation of LC-PUFA biosynthesis by facilitatingfadexpression, probably through targetinginsig1. To our knowledge, this is the first report of the participation of miR-33 in LC-PUFA biosynthesis in vertebrates.en_UK
dc.publisherSpringer Natureen_UK
dc.relationZhang Q, You C, Wang S, Dong Y, Monroig O, Tocher DR & Li Y (2016) The miR-33 gene is identified in a marine teleost: a potential role in regulation of LC-PUFA biosynthesis in Siganus canaliculatus. Scientific Reports, 6, Art. No.: 32909.
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
dc.subjectGene regulationen_UK
dc.titleThe miR-33 gene is identified in a marine teleost: a potential role in regulation of LC-PUFA biosynthesis in Siganus canaliculatusen_UK
dc.typeJournal Articleen_UK
dc.citation.jtitleScientific Reportsen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationComplex Systems - LEGACYen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationShantou Universityen_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorZhang, Qinghao|en_UK
local.rioxx.authorYou, Cuihong|en_UK
local.rioxx.authorWang, Shuqi|en_UK
local.rioxx.authorDong, Yewei|en_UK
local.rioxx.authorMonroig, Oscar|0000-0001-8712-0440en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorLi, Yuanyou|en_UK
local.rioxx.projectInternal Project|University of Stirling|
Appears in Collections:Aquaculture Journal Articles

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