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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: pRNA: NoRC-associated RNA of rRNA operons
Author(s): Wehner, Stefanie
Dorrich, Anja K
Ciba, Philipp
Wilde, Annegret
Marz, Manja
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Keywords: promoter-associated RNA
non-coding RNA
ribosomal RNA
gene silencing
Issue Date: 2014
Date Deposited: 26-Aug-2016
Citation: Wehner S, Dorrich AK, Ciba P, Wilde A & Marz M (2014) pRNA: NoRC-associated RNA of rRNA operons. RNA Biology, 11 (1), pp. 3-9.
Abstract: Promoter-associated RNAs (pRNAs) are a family of ~90–100 nt-long divergent RNAs overlapping the promoter of the rRNA (rDNA) operon. pRNA transcripts interact with TIP5, a component of the chromatin remodeling complex NoRC, which recruits enzymes for heterochromatin formation and mediates silencing of rRNA genes. Here we present a comprehensive analysis of pRNA homologs, including different versions per species, as result of in silico studies in available metazoan genome assemblies. Comparative sequence analysis and secondary structure prediction ended up in two possible secondary structures, which let us assume a possible dual function of pRNAs for regulation of rRNA operons. Furthermore, we validated parts of our computational predictions experimentally by RT-PCR and sequencing. A representative seed alignment of the pRNA family, annotated with possible secondary structures was released to the Rfam database.
DOI Link: 10.4161/rna.27448
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