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dc.contributor.authorLechner, Marcusen_UK
dc.contributor.authorNickel, Astrid Ien_UK
dc.contributor.authorWehner, Stefanieen_UK
dc.contributor.authorRiege, Konstantinen_UK
dc.contributor.authorWieseke, Nicolasen_UK
dc.contributor.authorBeckmann, Benedikt Men_UK
dc.contributor.authorHartmann, Roland Ken_UK
dc.contributor.authorManz, Manjaen_UK
dc.description.abstractBackground  The Aquificales are a diverse group of thermophilic bacteria that thrive in terrestrial and marine hydrothermal environments. They can be divided into the families Aquificaceae, Desulfurobacteriaceae and Hydrogenothermaceae. Although eleven fully sequenced and assembled genomes are available, only little is known about this taxonomic order in terms of RNA metabolism.  Results  In this work, we compare the available genomes, extend their protein annotation, identify regulatory sequences, annotate non-coding RNAs (ncRNAs) of known function, predict novel ncRNA candidates, show idiosyncrasies of the genetic decoding machinery, present two different types of transfer-messenger RNAs and variations of the CRISPR systems. Furthermore, we performed a phylogenetic analysis of the Aquificales based on entire genome sequences, and extended this by a classification among all bacteria using 16S rRNA sequences and a set of orthologous proteins.  Combining severalin silicofeatures (e.g. conserved and stable secondary structures, GC-content, comparison based on multiple genome alignments) with an in vivo dRNA-seq transcriptome analysis of Aquifex aeolicus, we predict roughly 100 novel ncRNA candidates in this bacterium.  Conclusions  We have here re-analyzed the Aquificales, a group of bacteria thriving in extreme environments, sharing the feature of a small, compact genome with a reduced number of protein and ncRNA genes. We present several classical ncRNAs and riboswitch candidates. By combining in silico analysis with dRNA-seq data of A. aeolicus we predict nearly 100 novel ncRNA candidates.en_UK
dc.publisherBioMed Centralen_UK
dc.relationLechner M, Nickel AI, Wehner S, Riege K, Wieseke N, Beckmann BM, Hartmann RK & Manz M (2014) Genomewide comparison and novel ncRNAs of Aquificales. BMC Genomics, 15, Art. No.: 522.
dc.rights© Lechner et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.en_UK
dc.titleGenomewide comparison and novel ncRNAs of Aquificalesen_UK
dc.typeJournal Articleen_UK
dc.citation.jtitleBMC Genomicsen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationPhilipps University of Marburgen_UK
dc.contributor.affiliationPhilipps University of Marburgen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationFriedrich Schiller University of Jenaen_UK
dc.contributor.affiliationUniversity Leipzigen_UK
dc.contributor.affiliationHumboldt University Berlinen_UK
dc.contributor.affiliationPhilipps University of Marburgen_UK
dc.contributor.affiliationFriedrich Schiller University of Jenaen_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorLechner, Marcus|en_UK
local.rioxx.authorNickel, Astrid I|en_UK
local.rioxx.authorWehner, Stefanie|0000-0002-3632-2584en_UK
local.rioxx.authorRiege, Konstantin|en_UK
local.rioxx.authorWieseke, Nicolas|en_UK
local.rioxx.authorBeckmann, Benedikt M|en_UK
local.rioxx.authorHartmann, Roland K|en_UK
local.rioxx.authorManz, Manja|en_UK
local.rioxx.projectInternal Project|University of Stirling|
Appears in Collections:Aquaculture Journal Articles

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