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dc.contributor.authorSalini, Michael Jen_UK
dc.contributor.authorWade, Nicholas Men_UK
dc.contributor.authorAraujo, Bruno Cen_UK
dc.contributor.authorTurchini, Giovanni Men_UK
dc.contributor.authorGlencross, Bretten_UK
dc.description.abstractA two part experiment was conducted to assess the response of barramundi (Lates calcarifer; initial weight=10.3±0.03g; mean±S.D.) fed one of five diets with varying eicosapentaenoic acid (diets 1, 5, 10, 15 and 20g/kg) or one of four diets with varying arachidonic acid (1, 6, 12, 18g/kg) against a fish oil control diet. After 6weeks of feeding, the addition of EPA or ARA did not impact on growth performance or feed utilisation. Analysis of the whole body fatty acids showed that these reflected those of the diets. The ARA retention demonstrated an inversely related curvilinear response to either EPA or ARA. The calculated marginal utilisation efficiencies of EPA and ARA were high (62.1 and 91.9% respectively) and a dietary ARA requirement was defined (0.012g/kg0.796/day). The partial cDNA sequences of genes regulating eicosanoid biosynthesis were identified in barramundi tissues, namely cyclooxygenase 1 (Lc COX1a, Lc COX1b), cyclooxygenase 2 (Lc COX2) and lipoxygenase (Lc ALOX-5). BothLc COX2andLc ALOX-5expression in the liver tissue were elevated in response to increasing dietary ARA, meanwhile expression levels ofLcCOX2and the mitochondrial fatty acid oxidation gene carnitine palmitoyltransferase 1 (LcCPT1a) were elevated in the kidney. A low level of EPA increased the expression ofLc COX1bin the liver. Consideration should be given to the EPA to ARA balance for juvenile barramundi in light of nutritionally inducible nature of the cyclooxygenase and lipoxygenase enzymes.en_UK
dc.relationSalini MJ, Wade NM, Araujo BC, Turchini GM & Glencross B (2016) Eicosapentaenoic Acid, Arachidonic Acid and Eicosanoid Metabolism in Juvenile Barramundi Lates calcarifer. Lipids, 51 (8), pp. 973-988.
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Publisher policy allows this work to be made available in this repository; The final publication is available at Springer via
dc.subjectArachidonic aciden_UK
dc.subjectGene expressionen_UK
dc.subjectMolecular cloningen_UK
dc.titleEicosapentaenoic Acid, Arachidonic Acid and Eicosanoid Metabolism in Juvenile Barramundi Lates calcariferen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[Defining the quantitative ARA and EPA_FINAL.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.affiliationDeakin Universityen_UK
dc.contributor.affiliationCSIRO Marine and Atmospheric Researchen_UK
dc.contributor.affiliationUniversity of Sao Pauloen_UK
dc.contributor.affiliationDeakin Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
Appears in Collections:Aquaculture Journal Articles

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