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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Identification of DLD, by immunoproteomic analysis and evaluation as a potential vaccine antigen against three Vibrio species in Epinephelus coioides
Author(s): Pang, Huanying
Chen, Liming
Hoare, Rowena
Huang, Yucong
Wu, Zaohe
Jian, Jichang
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Keywords: Vibrio alginolyticus
Vibrio harveyi
Vibrio parahaemolyticus
Cross immunogenic proteins
DLD (dihydrolipoamide dehydrogenase)
Issue Date: Feb-2016
Date Deposited: 8-Jul-2016
Citation: Pang H, Chen L, Hoare R, Huang Y, Wu Z & Jian J (2016) Identification of DLD, by immunoproteomic analysis and evaluation as a potential vaccine antigen against three Vibrio species in Epinephelus coioides. Vaccine, 34 (9), pp. 1225-1231.
Abstract: Vibrio spp. represent a serious threat to the culture of Epinephelus coioides (Orange-spotted Grouper) in Southeast Asia. In this study we used two-dimensional electrophoresis (2-DE) and Western blotting to identify common immunogenic proteins of Vibrio alginolyticus, Vibrio harveyi and Vibrio parahaemolyticus. Membranes were probed with orange-spotted grouper anti-V. alginolyticussera and accordingly 60, 58 and 48 immunogenic protein spots were detected. By matching analysis for the three Western blotting membranes, 6 cross immunogenic spots for the three Vibrio species were identified. They were Outer membrane protein W (OmpW), dihydrolipoamide dehydrogenase (DLD), succinate dehydrogenase flavoprotein subunit(SDHA), elongation factor Ts(Ts), peptide ABC transporter periplasmic peptide-binding protein and phosphoenolpyruvate carboxykinase(PEPCK). One of the proteins, DLD, was used to evaluate the cross protective function for E. coioides with a bacterial immunization and challenge method. The relative percent survival rate of E. coioides against V. alginolyticus ,V. harveyi and V. parahaemolyticus was 90%, 86% and 80%, respectively. This work may provide potential cross protective vaccine candidate antigens for three Vibrio species, and DLD may be considered as an effective cross-protective immunogen against three Vibrio species.
DOI Link: 10.1016/j.vaccine.2015.11.001
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