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dc.contributor.authorVera, LMen_UK
dc.contributor.authorMontoya, Anderen_UK
dc.contributor.authorPujante, Isabelen_UK
dc.contributor.authorPerez Sanchez, Jaumeen_UK
dc.contributor.authorCalduch-Giner, Josepen_UK
dc.contributor.authorMancera, Juan Miguelen_UK
dc.contributor.authorMoliner, Javieren_UK
dc.contributor.authorSanchez Vazquez, Francisco Jen_UK
dc.description.abstractSince fish show daily rhythms in most physiological functions, it should not be surprising that stressors may have different effects depending on the timing of exposure. In this study, we investigated the influence of time of day on the stress responses, at both physiological and cellular levels, in gilthead sea bream (Sparus aurataL.) submitted to air exposure for 30 s and then returned to their tank. One hour after air exposure, blood, hypothalamus and liver samples were taken. Six fish per experimental group (control and stressed) were sampled every 4 h during a 24-h cycle. Fish were fed in the middle of the light cycle (ML) and locomotor activity rhythms were recorded using infrared photocells to determine their daily activity pattern of behaviour, which showed a peak around feeding time in all fish. In the control group, cortisol levels did not show daily rhythmicity, whereas in the stressed fish, a daily rhythm of plasma cortisol was observed, being the average values higher than in the control group, with increased differences during the dark phase. Blood glucose showed daily rhythmicity in the control group but not in the stressed one which also showed higher values at all sampling points. In the hypothalamus of control fish, a daily rhythm ofcorticotropin-releasing hormone(crh) gene expression was observed, with the acrophase at the beginning of the light phase. However, in the stressed fish, this rhythm was abolished. The expression ofcrh-binding protein(crhbp) showed a peak at the end of the dark phase in the control group, whereas in the stressed sea bream, this peak was found at ML. Regarding hepatic gene expression of oxidative stress biomarkers: (i)cytochrome c oxidase 4showed daily rhythmicity in both control and stressed fish, with the acrophases located around ML, (ii)peroxiredoxin(prdx) 3 and5(prdx5) only presented daily rhythmicity of expression in the stressed fish, with the acrophase located at the beginning of the light cycle and (iii)uncoupling protein 1showed significant differences between sampling points only in the control group, with significantly higher expression at the beginning of the dark phase. Taken together, these results indicate that stress response in gilthead sea bream is time-dependent as cortisol level rose higher at night, and that different rhythmic mechanisms interplay in the control of neuroendocrine and cellular stress responses.en_UK
dc.publisherTaylor and Francisen_UK
dc.relationVera L, Montoya A, Pujante I, Perez Sanchez J, Calduch-Giner J, Mancera JM, Moliner J & Sanchez Vazquez FJ (2014) Acute stress response in gilthead sea bream (Sparus aurata L.) is time-of-day dependent: Physiological and oxidative stress indicators. Chronobiology International, 31 (9), pp. 1051-1061.
dc.rightsThis is an Accepted Manuscript of an article published by Taylor & Francis Group in Chronobiology International on 07 Aug 2014, available online:
dc.subjectcrh expressionen_UK
dc.subjectcrhbp expressionen_UK
dc.subjectdaily rhythmsen_UK
dc.subjectfish welfareen_UK
dc.subjectoxidative stress biomarkersen_UK
dc.titleAcute stress response in gilthead sea bream (Sparus aurata L.) is time-of-day dependent: Physiological and oxidative stress indicatorsen_UK
dc.typeJournal Articleen_UK
dc.citation.jtitleChronobiology Internationalen_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Murciaen_UK
dc.contributor.affiliationUniversity of Cadizen_UK
dc.contributor.affiliationSpanish National Research Council (CSIC)en_UK
dc.contributor.affiliationSpanish National Research Council (CSIC)en_UK
dc.contributor.affiliationUniversity of Cadizen_UK
dc.contributor.affiliationUniversity of Murciaen_UK
dc.contributor.affiliationUniversity of Murciaen_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorVera, LM|0000-0003-0999-055Xen_UK
local.rioxx.authorMontoya, Ander|en_UK
local.rioxx.authorPujante, Isabel|en_UK
local.rioxx.authorPerez Sanchez, Jaume|en_UK
local.rioxx.authorCalduch-Giner, Josep|en_UK
local.rioxx.authorMancera, Juan Miguel|en_UK
local.rioxx.authorMoliner, Javier|en_UK
local.rioxx.authorSanchez Vazquez, Francisco J|en_UK
local.rioxx.projectInternal Project|University of Stirling|
Appears in Collections:Aquaculture Journal Articles

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