Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/23337
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dc.contributor.authorReyes-Lopez, Felipe-
dc.contributor.authorRomeo, Jose S-
dc.contributor.authorVallejos-Vidal, Eva-
dc.contributor.authorReyes-Cerpa, Sebastian-
dc.contributor.authorSandino, Ana M-
dc.contributor.authorTort, Lluis-
dc.contributor.authorMacKenzie, Simon-
dc.contributor.authorImarai, Monica-
dc.date.accessioned2016-06-17T23:43:04Z-
dc.date.issued2015-11-
dc.identifier.urihttp://hdl.handle.net/1893/23337-
dc.description.abstractThis study aims to identify at the expression level the immune-related genes associated with IPN-susceptible and resistant phenotypes in Atlantic salmon full-sibling families. We have analyzed thirty full-sibling families infected by immersion with IPNV and then classified as resistant or susceptible using a multivariate survival analysis based on a gamma-Cox frailty model and the Kaplan–Meier mortality curves. In four families within each group head kidneys were pooled for real-time PCR and one-color salmon-specific oligonucleotide microarray (21K) analysis at day 1 and 5 post-infection. Transcripts involved in innate response (IL-6, IFN-α), antigen presentation (HSP-70, HSP-90, MHC-I), TH1 response (IL-12, IFN-γ, CRFB6), immunosuppression (IL-10, TGF-β1) and leukocyte activation and migration (CCL-19, CD18) showed a differential expression pattern between both phenotypes, except in IL-6. In susceptible families, except for IFN-γ, the expressions dropped to basal values at day 5 post-infection. In resistant families, unlike susceptible families, levels remained high or increased (except for IL-6) at day 5. Transcriptomic analysis showed that both families have a clear differential expression pattern, resulting in a marked down-regulation in immune related genes involved in innate response, complement system, antigen recognition and activation of immune response in IPN-resistant. Down-regulation of genes, mainly related to tissue differentiation and protein degradation metabolism, was also observed in resistant families. We have identified an immune-related gene patterns associated with susceptibility and resistance to IPNV infection of Atlantic salmon. This suggests that a limited immune response is associated with resistant fish phenotype to IPNV challenge while a highly inflammatory but short response is associated with susceptibility.en_UK
dc.language.isoen-
dc.publisherElsevier-
dc.relationReyes-Lopez F, Romeo JS, Vallejos-Vidal E, Reyes-Cerpa S, Sandino AM, Tort L, MacKenzie S & Imarai M (2015) Differential immune gene expression profiles in susceptible and resistant full-sibling families of Atlantic salmon (Salmo salar) challenged with infectious pancreatic necrosis virus (IPNV), Developmental and Comparative Immunology, 53 (1), pp. 210-221.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.subjectIPN-Resistanceen_UK
dc.subjectFull-sibling familiesen_UK
dc.subjectSalmo salaren_UK
dc.subjectTranscriptomicsen_UK
dc.subjectMicroarraysen_UK
dc.subjectImmune responseen_UK
dc.titleDifferential immune gene expression profiles in susceptible and resistant full-sibling families of Atlantic salmon (Salmo salar) challenged with infectious pancreatic necrosis virus (IPNV)en_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31T00:00:00Z-
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.identifier.doihttp://dx.doi.org/10.1016/j.dci.2015.06.017-
dc.identifier.pmid26123889-
dc.citation.jtitleDevelopmental and Comparative Immunology-
dc.citation.issn0145-305X-
dc.citation.volume53-
dc.citation.issue1-
dc.citation.spage210-
dc.citation.epage221-
dc.citation.publicationstatusPublished-
dc.citation.peerreviewedRefereed-
dc.type.statusPublisher version (final published refereed version)-
dc.author.emailsimon.mackenzie@stir.ac.uk-
dc.citation.date27/06/2015-
dc.contributor.affiliationUniversitat Autonoma de Barcelona-
dc.contributor.affiliationUniversity of Santiago-
dc.contributor.affiliationUniversity of Santiago-
dc.contributor.affiliationUniversity of Santiago-
dc.contributor.affiliationUniversity of Santiago-
dc.contributor.affiliationUniversitat Autonoma de Barcelona-
dc.contributor.affiliationAquaculture-
dc.contributor.affiliationUniversity of Santiago-
dc.rights.embargoterms2999-12-31-
dc.rights.embargoliftdate2999-12-31-
dc.identifier.isi000360772900023-
Appears in Collections:Aquaculture Journal Articles

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