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dc.contributor.authorOboh, Angelaen_UK
dc.contributor.authorBetancor, Mónica Ben_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorMonroig, Oscaren_UK
dc.description.abstractFish differ in their capacity for endogenous synthesis of long-chain (C20-24) polyunsaturated fatty acids (LC-PUFA) from dietary C18 precursors (α-linolenic and linoleic acids). Understanding this capacity is of benefit to fish feed formulation. This, together with the importance of fish as the primary source of omega-3 LC-PUFA in the human diet has necessitated the rigorous study of the biochemical and molecular mechanisms involved in the LC-PUFA biosynthesis pathway in fish species. Studies have shown the potential of a species for LC-PUFA biosynthesis is associated with the complement and function of fatty acyl desaturase (fads) and elongase of very long chain fatty acid (elovl) gene it possesses. The present study therefore aimed to investigate these genes in the African catfish (Clarias gariepinus), the most commercially important farmed fish species in Sub-Saharan Africa. A fads2 and an elovl2 cDNA were cloned containing open reading frames (ORF) of 1338 base pair (bp) and 864 bp specifying proteins of 445 and 287 amino acids, respectively. Functional characterisation by heterologous expression in yeast showed that the Fads2 was bifunctional with ∆5∆6 activities catalysing the desaturation of both 18:3n-3 and 20:4n-3 and their corresponding n-6 fatty acids, 18:2n-6 and 20:3n-6. The Elovl2 showed activity towards C18, C20 and C22 PUFA with highest activity towards C20 and C22 PUFA. Tissue expression analysis showed a typical freshwater species expression pattern; higher expression in the liver compared to brain and all other tissues with the exception of elovl5 which showed highest expression in the intestine. Consistent with feeding studies of typical freshwater fish species that show their essential fatty acid requirement can be satisfied by dietary C18 PUFA, the present study confirms that the LC-PUFA biosynthesis pathway is active in the African catfish C. gariepinus.en_UK
dc.relationOboh A, Betancor MB, Tocher DR & Monroig O (2016) Biosynthesis of long-chain polyunsaturated fatty acids in the African catfish Clarias gariepinus: Molecular cloning and functional characterisation of fatty acyl desaturase (fads2) and elongase (elovl2) cDNAs7. Aquaculture, 462, pp. 70-79.
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Oboh A, Betancor M, Tocher DR & Monroig O (2016) Biosynthesis of long-chain polyunsaturated fatty acids in the African catfish Clarias gariepinus: Molecular cloning and functional characterisation of fatty acyl desaturase (fads2) and elongase (elovl2) cDNAs, Aquaculture, 462, pp. 70-79. DOI: 10.1016/j.aquaculture.2016.05.018 © 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
dc.subjectClarias gariepinusen_UK
dc.subjectessential fatty acidsen_UK
dc.subjectlong-chain polyunsaturated fatty acidsen_UK
dc.titleBiosynthesis of long-chain polyunsaturated fatty acids in the African catfish Clarias gariepinus: Molecular cloning and functional characterisation of fatty acyl desaturase (fads2) and elongase (elovl2) cDNAs7en_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[Oboh et al Aquaculture 2016 STORRE.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationComplex Systems - LEGACYen_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorOboh, Angela|en_UK
local.rioxx.authorBetancor, Mónica B|0000-0003-1626-7458en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorMonroig, Oscar|0000-0001-8712-0440en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameOboh et al Aquaculture 2016 STORRE.pdfen_UK
Appears in Collections:Aquaculture Journal Articles

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