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dc.contributor.authorLynsdale, Carly Len_UK
dc.contributor.authordos Santos, Diogo J Francoen_UK
dc.contributor.authorHayward, Adamen_UK
dc.contributor.authorMar, Khyne Uen_UK
dc.contributor.authorMar, Khyne Uen_UK
dc.contributor.authorHtut, Winen_UK
dc.contributor.authorAung, Htoo Htooen_UK
dc.contributor.authorSoe, Aung Thuraen_UK
dc.contributor.authorLummaa, Virpien_UK
dc.description.abstractThe quantitative assessment of parasite infection is necessary to measure, manage and reduce infection risk in both wild and captive animal populations. Traditional faecal flotation methods which aim to quantify parasite burden, such as the McMaster egg counting technique, are widely used in veterinary medicine, agricultural management and wildlife parasitology. Although many modifications to the McMaster method exist, few account for systematic variation in parasite egg output which may lead to inaccurate estimations of infection intensity through faecal egg counts (FEC). To adapt the McMaster method for use in sampling Asian elephants (Elephas maximus), we tested a number of possible sources of error regarding faecal sampling, focussing on helminth eggs and using a population of over 120 semi-captive elephants distributed across northern Myanmar. These included time of day of defecation, effects of storage in 10% formalin and 10% formol saline and variation in egg distribution between and within faecal boluses. We found no significant difference in the distribution of helminth eggs within faecal matter or for different defecation times, however, storage in formol saline and formalin significantly decreased egg recovery. This is the first study to analyse several collection and storage aspects of a widely-used traditional parasitology method for helminth parasites ofE.maximususing known host individuals. We suggest that for the modified McMaster technique, a minimum of one fresh sample per elephant collected from any freshly produced bolus in the total faecal matter and at any point within a 7.5h time period (7.30am–2.55pm) will consistently represent parasite load. This study defines a protocol which may be used to test pre-analytic factors and effectively determine infection load in species which produce large quantities of vegetative faeces, such as non-ruminant megaherbivores.en_UK
dc.relationLynsdale CL, dos Santos DJF, Hayward A, Mar KU, Mar KU, Htut W, Aung HH, Soe AT & Lummaa V (2015) A standardised faecal collection protocol for intestinal helminth egg counts in Asian elephant, Elephas maximus. International Journal for Parasitology: Parasites and Wildlife, 4 (3), pp. 307-315.
dc.rightsCopyright 2015 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology. This is an open access article under the CC BY license (
dc.subjectFaecal egg counten_UK
dc.subjectSampling methoden_UK
dc.titleA standardised faecal collection protocol for intestinal helminth egg counts in Asian elephant, Elephas maximusen_UK
dc.typeJournal Articleen_UK
dc.citation.jtitleInternational Journal for Parasitology: Parasites and Wildlifeen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationUniversity of Sheffielden_UK
dc.contributor.affiliationUniversity of Lisbonen_UK
dc.contributor.affiliationBiological and Environmental Sciencesen_UK
dc.contributor.affiliationZoological Society of Londonen_UK
dc.contributor.affiliationUniversity of Sheffielden_UK
dc.contributor.affiliationMinistry of Environmental Conservation and Forestryen_UK
dc.contributor.affiliationMinistry of Environmental Conservation and Forestryen_UK
dc.contributor.affiliationMinistry of Environmental Conservation and Forestryen_UK
dc.contributor.affiliationUniversity of Sheffielden_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorLynsdale, Carly L|en_UK
local.rioxx.authordos Santos, Diogo J Franco|en_UK
local.rioxx.authorHayward, Adam|0000-0001-6953-7509en_UK
local.rioxx.authorMar, Khyne U|en_UK
local.rioxx.authorMar, Khyne U|en_UK
local.rioxx.authorHtut, Win|en_UK
local.rioxx.authorAung, Htoo Htoo|en_UK
local.rioxx.authorSoe, Aung Thura|en_UK
local.rioxx.authorLummaa, Virpi|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameLynsdale et al_IJPPW_2015.pdfen_UK
Appears in Collections:Biological and Environmental Sciences Journal Articles

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