Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/22825
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dc.contributor.authorTejera, Noemi-
dc.contributor.authorVauzour, David-
dc.contributor.authorBetancor, Monica-
dc.contributor.authorSayanova, Olga-
dc.contributor.authorUsher, Sarah-
dc.contributor.authorCochard, Marianne-
dc.contributor.authorRigby, Neil-
dc.contributor.authorRuiz-Lopez, Noemi-
dc.contributor.authorMenoyo, David-
dc.contributor.authorTocher, Douglas R-
dc.contributor.authorNapier, Johnathan A-
dc.contributor.authorMinihane, Anne Marie-
dc.date.accessioned2017-01-19T01:35:39Z-
dc.date.available2017-01-19T01:35:39Z-
dc.date.issued2016-02-01-
dc.identifier.urihttp://hdl.handle.net/1893/22825-
dc.description.abstractBackground: Fish currently supplies only 40% of the eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) required to allow all individuals globally to meet the minimum intake recommendation of 500 mg/d. Therefore, alternative sustainable sources are needed.  Objective: The main objective was to investigate the ability of genetically engineeredCamelina sativa(20% EPA) oil (CO) to enrich tissue EPA and DHA relative to an EPA-rich fish oil (FO) in mammals.  Methods: Six-week-old male C57BL/6J mice were fed for 10 wk either a palm oil–containing control (C) diet or diets supplemented with EPA-CO or FO, with the C, low-EPA CO (COL), high-EPA CO (COH), low-EPA FO (FOL), and high-EPA FO (FOH) diets providing 0, 0.4, 3.4, 0.3, and 2.9 g EPA/kg diet, respectively. Liver, muscle, and brain were collected for fatty acid analysis, and blood glucose and serum lipids were quantified. The expression of selected hepatic genes involved in EPA and DHA biosynthesis and in modulating their cellular impact was determined.  Results: The oils were well tolerated, with significantly greater weight gain in the COH and FOH groups relative to the C group (P< 0.001). Significantly lower (36–38%) blood glucose concentrations were evident in the FOH and COH mice relative to C mice (P< 0.01). Hepatic EPA concentrations were higher in all EPA groups relative to the C group (P< 0.001), with concentrations of 0.0, 0.4, 2.9, 0.2, and 3.6 g/100 g liver total lipids in the C, COL, COH, FOL, and FOH groups, respectively. Comparable dose-independent enrichments of liver DHA were observed in mice fed CO and FO diets (P< 0.001). Relative to the C group, lower fatty acid desaturase 1 (Fads1) expression (P< 0.005) was observed in the COH and FOH groups. Higher fatty acid desaturase 2 (Fads2), peroxisome proliferator–activated receptor α (Ppara), and peroxisome proliferator–activated receptor γ (Pparg) (P< 0.005) expressions were induced by CO. No impact of treatment on liver X receptor α (Lxra) or sterol regulatory element-binding protein 1c (Srebp1c) was evident.  Conclusions: Oil from transgenicCamelinais a bioavailable source of EPA in mice. These data provide support for the future assessment of this oil in a human feeding trial.en_UK
dc.language.isoen-
dc.publisherAmerican Society for Nutrition-
dc.relationTejera N, Vauzour D, Betancor M, Sayanova O, Usher S, Cochard M, Rigby N, Ruiz-Lopez N, Menoyo D, Tocher DR, Napier JA & Minihane AM (2016) A transgenic Camelina sativa seed oil effectively replaces fish oil as a dietary source of eicosapentaenoic acid in mice, Journal of Nutrition, 146 (2), pp. 227-235.-
dc.rightsThis is an open access article distributed under the CC-BY license (http://creativecommons.org/licenses/by/3.0/).-
dc.subjectn–3 PUFAen_UK
dc.subjectEPAen_UK
dc.subjectDHAen_UK
dc.subjectCamelina oilen_UK
dc.subjectfish oilen_UK
dc.subjectsustainabilityen_UK
dc.subjectdesaturationen_UK
dc.subjectFadsen_UK
dc.subjecttransgenicen_UK
dc.subjectTG sn-2en_UK
dc.titleA transgenic Camelina sativa seed oil effectively replaces fish oil as a dietary source of eicosapentaenoic acid in miceen_UK
dc.typeJournal Articleen_UK
dc.identifier.doihttp://dx.doi.org/10.3945/jn.115.223941-
dc.identifier.pmid26791554-
dc.citation.jtitleJournal of Nutrition-
dc.citation.issn0022-3166-
dc.citation.volume146-
dc.citation.issue2-
dc.citation.spage227-
dc.citation.epage235-
dc.citation.publicationstatusPublished-
dc.citation.peerreviewedRefereed-
dc.type.statusPublisher version (final published refereed version)-
dc.author.emaild.r.tocher@stir.ac.uk-
dc.citation.date20/01/2016-
dc.contributor.affiliationUniversity of East Anglia-
dc.contributor.affiliationUniversity of East Anglia-
dc.contributor.affiliationAquaculture-
dc.contributor.affiliationRothamsted Research-
dc.contributor.affiliationRothamsted Research-
dc.contributor.affiliationUniversity of East Anglia-
dc.contributor.affiliationInstitute of Food Research-
dc.contributor.affiliationTechnical University of Madrid-
dc.contributor.affiliationUniversidad Politécnica de Madrid-
dc.contributor.affiliationAquaculture-
dc.contributor.affiliationRothamsted Research-
dc.contributor.affiliationUniversity of East Anglia-
dc.identifier.isi000369557800007-
Appears in Collections:Aquaculture Journal Articles

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