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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: A scanning electron microscope technique for studying the sclerites of Cichlidogyrus
Author(s): Fannes, Wouter
Vanhove, Maarten P M
Huyse, Tine
Paladini, Giuseppe
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Keywords: Platyhelminthes
Male apparatus
Issue Date: May-2015
Date Deposited: 27-Jan-2016
Citation: Fannes W, Vanhove MPM, Huyse T & Paladini G (2015) A scanning electron microscope technique for studying the sclerites of Cichlidogyrus. Parasitology Research, 114 (5), pp. 2031-2034.
Abstract: The genusCichlidogyrus(Monogenea: Ancyrocephalidae) includes more than 90 species, most of which are gill parasites of African cichlid fishes.Cichlidogyrushas been studied extensively in recent years, but scanning electron microscope (SEM) investigations of the isolated hard parts have not yet been undertaken. In this paper, we describe a method for isolating and scanning the sclerites of individualCichlidogyrusworms. Twenty-year-old, formol-fixed specimens ofCichlidogyrus casuarinuswere subjected to proteinase K digestion in order to release the sclerites from the surrounding soft tissues. SEM micrographs of the haptoral sclerites and the male copulatory organ are presented. The ability to digest formol-fixed specimens makes this method a useful tool for the study of historical museum collections.
DOI Link: 10.1007/s00436-015-4446-7
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