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dc.contributor.authorXie, Dizhi-
dc.contributor.authorWang, Shuqi-
dc.contributor.authorYou, Cuihong-
dc.contributor.authorChen, Fang-
dc.contributor.authorTocher, Douglas R-
dc.contributor.authorLi, Yuanyou-
dc.description.abstractThe present study aimed to characterize the influence of salinity on the biosynthesis of long-chain polyunsaturated fatty acid (LC-PUFA) in rabbitfishSiganus canaliculatus. An eight-week feeding trial was performed in rabbitfish juveniles with diets containing fish oil (FO) or a blend of vegetable oils (perilla and Canola oils, VO) at two salinities, 32 and 10ppt. The whole-body fatty acid mass balance (FAMB) method was used to evaluate thein vivoLC-PUFA biosynthetic activities, and the hepatic mRNA levels of Δ4 and Δ6/Δ5 fatty acyl desaturases (Fad) and elongase of very long-chain fatty acids (Elovl5) genes were determined by real-time quantitative PCR. The results showed that theex novoproduction of LC-PUFA in fish receiving the VO diet was significantly higher than fish fed the FO diet at both salinities. Furthermore, LC-PUFA production at 10ppt salinity was significantly higher than that at 32ppt salinity in the VO dietary groups, whereas no effect of salinity was found in the FO dietary groups. Consistent with this, the calculated apparentin vivodesaturation and elongation activities were also higher in VO and low-salinity treatments. In addition, higher levels ofΔ4andΔ6/Δ5 fadsmRNA expression were obtained at low salinity, which was consistent with the calculated enzyme activities. In contrast, the expression ofelovl5was lower than that offads,and the levels were not consistent with the elongase activity. The results suggest that ambient salinity may affect the activity of the LC-PUFA biosynthetic pathway in rabbitfish through regulating fatty acyl desaturase and elongase activities, partly through a transcriptional control mechanism in the case of desaturases.en_UK
dc.relationXie D, Wang S, You C, Chen F, Tocher DR & Li Y (2015) Characteristics of LC-PUFA biosynthesis in marine herbivorous teleost Siganus canaliculatus under different ambient salinities, Aquaculture Nutrition, 21 (5), pp. 541-551.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.subjectfatty acid mass balanceen_UK
dc.titleCharacteristics of LC-PUFA biosynthesis in marine herbivorous teleost Siganus canaliculatus under different ambient salinitiesen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.citation.jtitleAquaculture Nutrition-
dc.type.statusPublisher version (final published refereed version)-
dc.contributor.affiliationShantou University-
dc.contributor.affiliationShantou University-
dc.contributor.affiliationShantou University-
dc.contributor.affiliationShantou University-
dc.contributor.affiliationShantou University-
Appears in Collections:Aquaculture Journal Articles

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