Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/22759
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dc.contributor.authorMonaghan, Sean Jen_UK
dc.contributor.authorThompson, Kim Den_UK
dc.contributor.authorBron, James Wen_UK
dc.contributor.authorBergmann, Sven Men_UK
dc.contributor.authorJung, Tae Sen_UK
dc.contributor.authorAoki, Takashien_UK
dc.contributor.authorMuir, K Fionaen_UK
dc.contributor.authorDauber, Malteen_UK
dc.contributor.authorReiche, Svenen_UK
dc.contributor.authorChee, Dianaen_UK
dc.contributor.authorChong, Shin Men_UK
dc.contributor.authorChen, Jingen_UK
dc.contributor.authorAdams, Alexandraen_UK
dc.date.accessioned2016-02-02T00:22:45Z-
dc.date.available2016-02-02T00:22:45Z-
dc.date.issued2016-01-08en_UK
dc.identifier.other8en_UK
dc.identifier.urihttp://hdl.handle.net/1893/22759-
dc.description.abstractCyprinid herpesvirus 3 (CyHV-3), also called koi herpesvirus (KHV), is the aetiological agent of a fatal disease in carp and koi (Cyprinus carpioL.), referred to as koi herpesvirus disease. The virus contains at least 40 structural proteins, of which few have been characterised with respect to their immunogenicity. Indirect immunofluorescence assays (IFAs) using two epitope-specific monoclonal antibodies (MAbs) were used to examine the expression kinetics of two potentially immunogenic and diagnostically relevant viral antigens, an envelope glycoprotein and a capsid-associated protein. The rate of expression of these antigens was determined following a time-course of infection in two CyHV-3 susceptible cell lines. The results were quantified using an IFA, performed in microtitre plates, and image analysis was used to analyse confocal micrographs, enabling measurement of differential virus-associated fluorescence and nucleus-associated fluorescence from stacks of captured scans. An 8-tenfold increase in capsid-associated protein expression was observed during the first 5days post-infection compared to a ≤2-fold increase in glycoprotein expression. A dominant protein of ~100kDa reacted with the capsid-associated MAb (20F10) in western blot analysis. This band was also recognised by sera obtained from carp infected with CyHV-3, indicating that this capsid-associated protein is produced in abundance during infection in vitro and is immunogenic to carp. Mass spectrometry carried out on this protein identified it as a previously uncharacterised product of open reading frame 84. This abundantly expressed and immunogenic capsid-associated antigen may be a useful candidate for KHV serological diagnostics.en_UK
dc.language.isoenen_UK
dc.publisherBioMed Centralen_UK
dc.relationMonaghan S, Thompson K, Bron J, Bergmann S, Jung T, Aoki T, Muir K, Dauber M, Reiche S, Chee D, Chong SM, Chen J & Adams A (2016) Expression of immunogenic structural proteins of cyprinid herpesvirus 3 in vitro assessed using immunofluorescence, Veterinary Research, 47 (1), Art. No.: 8. https://doi.org/10.1186/s13567-015-0297-6.en_UK
dc.rights© 2016 Monaghan et al. Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en_UK
dc.titleExpression of immunogenic structural proteins of cyprinid herpesvirus 3 in vitro assessed using immunofluorescenceen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1186/s13567-015-0297-6en_UK
dc.identifier.pmid26742989en_UK
dc.citation.jtitleVeterinary Researchen_UK
dc.citation.issn1297-9716en_UK
dc.citation.issn0928-4249en_UK
dc.citation.volume47en_UK
dc.citation.issue1en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.funderMSD Animal Healthen_UK
dc.author.emails.j.monaghan@stir.ac.uken_UK
dc.citation.date08/01/2016en_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationFriedrich-Loeffler Instituten_UK
dc.contributor.affiliationGyeongsang National Universityen_UK
dc.contributor.affiliationTokyo University of Fisheriesen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationFriedrich-Loeffler Instituten_UK
dc.contributor.affiliationFriedrich-Loeffler Instituten_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationAgri-Food and Veterinary Authority of Singaporeen_UK
dc.contributor.affiliationAgri-Food and Veterinary Authority of Singaporeen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isi000367793200006en_UK
dc.identifier.scopusid2-s2.0-84953223090en_UK
dc.identifier.wtid580039en_UK
dc.contributor.orcid0000-0002-7692-7756en_UK
dc.contributor.orcid0000-0003-3544-0519en_UK
dc.date.accepted2015-05-10en_UK
dc.date.firstcompliantdepositdate2016-01-20en_UK
dc.description.refREF Compliant by Deposit in Stirling's Repositoryen_UK
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