Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/22717
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dc.contributor.authorLi, Songlinen_UK
dc.contributor.authorMonroig, Óscaren_UK
dc.contributor.authorNavarro, Juan Carlosen_UK
dc.contributor.authorYuan, Yuhuien_UK
dc.contributor.authorXu, Weien_UK
dc.contributor.authorMai, Kangsenen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorAi, Qinghuien_UK
dc.date.accessioned2017-02-09T22:54:52Z-
dc.date.available2017-02-09T22:54:52Z-
dc.date.issued2017-02en_UK
dc.identifier.urihttp://hdl.handle.net/1893/22717-
dc.description.abstractElongase of very long-chain fatty acids (Elovl) 4 probably plays a crucial role in marine fish species, where lack of Elovl2 has been considered as one possible reason for their low long-chain polyunsaturated fatty acids' (LC-PUFAs) biosynthetic capability. Elongase of very long-chain fatty acids 4 is the most recent member of the Elovl family that has been investigated in fish. Here, we report the molecular cloning and functional characterization of putativeelovl4cDNA isolated from marine teleost,Epinephelus coioides, and its expression in response to dietary n-3 LC-PUFA and docosahexaenoic acid (DHA) to eicosapentaenoic acid (EPA) ratio. Theelovl4cDNA of grouper was 2341bp including 301bp of 5′-untranslated region (UTR), 918bp of the coding region that encodes 305 amino acids (AA) and 1122bp of 3′UTR. Heterologous expression in yeast demonstrated that grouper Elovl4 could elongate saturated fatty acids (FA), especially 24:0 and 26:0, up to 36:0. Also, grouper Elovl4 effectively converted C20 and C22 polyunsaturated FAs to elongated polyenoic products up to C36. Tissue distribution analysis revealed that Elovl4 were widely transcribed in various tissues with the highest level in eye, brain and testis as described in other teleosts. The transcript level ofelovl4was significantly affected by dietary n-3 LC-PUFA and high LC-PUFA level repressess its expression. However, the ratio of DHA to EPA had no significant influence on its expression. These results may contribute to better understanding the LC-PUFA biosynthetic pathway in this fish species.en_UK
dc.language.isoenen_UK
dc.publisherWiley-Blackwellen_UK
dc.relationLi S, Monroig Ó, Navarro JC, Yuan Y, Xu W, Mai K, Tocher DR & Ai Q (2017) Molecular Cloning and functional characterization of a putative Elovl4 gene and its expression in response to dietary fatty acid profiles in orange-spotted grouper Epinephelus coioides, Aquaculture Research, 48 (2), pp. 537-552. https://doi.org/10.1111/are.12902.en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is the peer reviewed version of the following article: Li, S., Monroig, Ó., Navarro, J. C., Yuan, Y., Xu, W., Mai, K., Tocher, D. R. and Ai, Q. (2017), Molecular cloning and functional characterization of a putative Elovl4 gene and its expression in response to dietary fatty acid profiles in orange-spotted grouper Epinephelus coioides. Aquac Res, 48: 537–552, which has been published in final form at https://doi.org/10.1111/are.12902. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.en_UK
dc.subjectElovl4en_UK
dc.subjectcloningen_UK
dc.subjectfunctional characterizationen_UK
dc.subjectnutrition regulationen_UK
dc.subjectorange-spotted grouperen_UK
dc.titleMolecular Cloning and functional characterization of a putative Elovl4 gene and its expression in response to dietary fatty acid profiles in orange-spotted grouper Epinephelus coioidesen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[Li et al 2015 (Qingdao) STORRE.pdf] : The publisher requires an embargo of 12 months after formal publicationen_UK
dc.identifier.doi10.1111/are.12902en_UK
dc.citation.jtitleAquaculture Researchen_UK
dc.citation.issn1365-2109en_UK
dc.citation.issn1355-557Xen_UK
dc.citation.volume48en_UK
dc.citation.issue2en_UK
dc.citation.spage537en_UK
dc.citation.epage552en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emailoscar.monroig@stir.ac.uken_UK
dc.citation.date03/10/2015en_UK
dc.contributor.affiliationOcean University of Chinaen_UK
dc.contributor.affiliationComplex Systems - LEGACYen_UK
dc.contributor.affiliationInstitute of Aquaculture Torre de la Salen_UK
dc.contributor.affiliationOcean University of Chinaen_UK
dc.contributor.affiliationOcean University of Chinaen_UK
dc.contributor.affiliationOcean University of Chinaen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationOcean University of Chinaen_UK
dc.identifier.isi000391960400016en_UK
dc.identifier.scopusid2-s2.0-84947997868en_UK
dc.identifier.wtid580452en_UK
dc.contributor.orcid0000-0001-8712-0440en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dc.date.firstcompliantdepositdate2016-01-13en_UK
Appears in Collections:Aquaculture Journal Articles

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