|Appears in Collections:||Aquaculture Journal Articles|
|Peer Review Status:||Refereed|
|Title:||Establishment of a minigenome system for oropouche virus reveals the S genome segment to be significantly longer than reported previously|
|Author(s):||Acrani, Gustavo Olszanski|
Tilston-Lunel, Natasha L
Silva, Daisy E A
Nunes, Marcio R T
Elliott, Richard M
|Citation:||Acrani GO, Tilston-Lunel NL, Spiegel M, Weidmann M, Dilcher M, Silva DEA, Nunes MRT & Elliott RM (2015) Establishment of a minigenome system for oropouche virus reveals the S genome segment to be significantly longer than reported previously. Journal of General Virology, 96 (3), pp. 513-523. https://doi.org/10.1099/jgv.0.000005|
|Abstract:||Oropouche virus (OROV) is a medically important orthobunyavirus, which causes frequent outbreaks of a febrile illness in the northern parts of Brazil. However, despite being the cause of an estimated half a million human infections since its first isolation in Trinidad in 1955, details of the molecular biology of this tripartite, negative-sense RNA virus remain limited. We have determined the complete nucleotide sequence of the Brazilian prototype strain of OROV, BeAn 19991, and found a number of differences compared with sequences in the database. Most notable were that the S segment contained an additional 204 nt at the 3′ end and that there was a critical nucleotide mismatch at position 9 within the base-paired terminal panhandle structure of each genome segment. In addition, we obtained the complete sequence of the Trinidadian prototype strain TRVL-9760 that showed similar characteristics to the BeAn 19991 strain. By using a T7 RNA polymerase-driven minigenome system, we demonstrated that cDNA clones of the BeAn 19991 L and S segments expressed functional proteins, and also that the newly determined terminal untranslated sequences acted as functional promoters in the minigenome assay. By co-transfecting a cDNA to the viral glycoproteins, virus-like particles were generated that packaged a minigenome and were capable of infecting naive cells.|
|Rights:||This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Publisher policy allows this work to be made available in this repository. Published in Journal of General Virology by Microbiology Society. Acrani GO, Tilston-Lunel NL, Spiegel M, Weidmann M, Dilcher M, Silva DEA, Nunes MRT & Elliott RM (2015) Establishment of a minigenome system for oropouche virus reveals the S genome segment to be significantly longer than reported previously, Journal of General Virology, 96 (3), pp. 513-523 The original publication is available at: http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000005|
|Acrani et al_JGV_2015.pdf||Fulltext - Accepted Version||234.88 kB||Adobe PDF||View/Open|
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