Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/22290
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dc.contributor.authorSilva, Sandro Pen_UK
dc.contributor.authorDilcher, Meiken_UK
dc.contributor.authorWeber, Franziskaen_UK
dc.contributor.authorHufert, Frank Ten_UK
dc.contributor.authorWeidmann, Manfreden_UK
dc.contributor.authorCardoso, Jedson Fen_UK
dc.contributor.authorCarvalho, Valeria Len_UK
dc.contributor.authorChang, Jannifer Oen_UK
dc.contributor.authorMartins, Livia Cen_UK
dc.contributor.authorLima, Clayton P Sen_UK
dc.contributor.authorSilva, Daisy E Aen_UK
dc.contributor.authorVianez-Junior, Joao L S Gen_UK
dc.contributor.authorPopov, Vsevolod Len_UK
dc.contributor.authorTravassos da Rosa, Amelia P Aen_UK
dc.contributor.authorTesh, Robert Ben_UK
dc.contributor.authorVasconcelos, Pedro F Cen_UK
dc.contributor.authorNunes, Marcio R Ten_UK
dc.date.accessioned2015-10-02T23:12:55Z-
dc.date.available2015-10-02T23:12:55Z-
dc.date.issued2014-10en_UK
dc.identifier.urihttp://hdl.handle.net/1893/22290-
dc.description.abstractThe Orbivirus genus of the family Reoviridae is comprised of 22 virus species including the Changuinola virus (CGLV) serogroup. The complete genome sequences of 13 CGLV serotypes isolated between 1961 and 1988 from distinct geographic areas of the Brazilian Amazon region were obtained. All viral sequences were obtained from single-passaged CGLV strains grown in Vero cells. CGLVs are the only orbiviruses known to be transmitted by phlebotomine sandflies. Ultrastructure and molecular analyses by electron microscopy and gel electrophoresis, respectively revealed viral particles with typical orbivirus size and morphology, as well as the presence of a segmented genome with 10 segments. Full-length nucleotide sequencing of each of the ten RNA segments of the 13 CGLV serotypes provided basic information regarding the genome organization, encoded proteins and genetic traits. Segment 2 (encoding VP2) of the CGLV is uncommonly larger in comparison to those found in other orbiviruses and shows varying sizes even among different CGLV serotypes. Phylogenetic analyses support previous serologic findings, which indicated that CGLV constitutes a separate serogroup within the genus Orbivirus. In addition, 6 out of 13 analyzed CGLV serotypes show reassortment of their genome segments.en_UK
dc.language.isoenen_UK
dc.publisherSociety for General Microbiologyen_UK
dc.relationSilva SP, Dilcher M, Weber F, Hufert FT, Weidmann M, Cardoso JF, Carvalho VL, Chang JO, Martins LC, Lima CPS, Silva DEA, Vianez-Junior JLSG, Popov VL, Travassos da Rosa APA, Tesh RB, Vasconcelos PFC & Nunes MRT (2014) Genetic and biological characterization of selected Changuinola viruses (Reoviridae, Orbivirus) from Brazil. Journal of General Virology, 95 (10), pp. 2251-2259. https://doi.org/10.1099/vir.0.064691-0en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectArbovirusesen_UK
dc.subjectChanguinola virusen_UK
dc.subjectOrbivirusen_UK
dc.subjectreoviridaeen_UK
dc.titleGenetic and biological characterization of selected Changuinola viruses (Reoviridae, Orbivirus) from Brazilen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate3000-01-01en_UK
dc.rights.embargoreason[Silva et al_JGV_2014.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1099/vir.0.064691-0en_UK
dc.identifier.pmid24986085en_UK
dc.citation.jtitleJournal of General Virologyen_UK
dc.citation.issn1465-2099en_UK
dc.citation.issn0022-1317en_UK
dc.citation.volume95en_UK
dc.citation.issue10en_UK
dc.citation.spage2251en_UK
dc.citation.epage2259en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailm.w.weidmann@stir.ac.uken_UK
dc.contributor.affiliationEvandro Chagas Instituteen_UK
dc.contributor.affiliationGeorg-August University Gottingenen_UK
dc.contributor.affiliationGeorg-August University Gottingenen_UK
dc.contributor.affiliationGeorg-August University Gottingenen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationEvandro Chagas Instituteen_UK
dc.contributor.affiliationEvandro Chagas Instituteen_UK
dc.contributor.affiliationEvandro Chagas Instituteen_UK
dc.contributor.affiliationEvandro Chagas Instituteen_UK
dc.contributor.affiliationEvandro Chagas Instituteen_UK
dc.contributor.affiliationEvandro Chagas Instituteen_UK
dc.contributor.affiliationEvandro Chagas Instituteen_UK
dc.contributor.affiliationUniversity of Texas Medical Branchen_UK
dc.contributor.affiliationUniversity of Texas Medical Branchen_UK
dc.contributor.affiliationUniversity of Texas Medical Branchen_UK
dc.contributor.affiliationEvandro Chagas Instituteen_UK
dc.contributor.affiliationEvandro Chagas Instituteen_UK
dc.identifier.isiWOS:000344911900016en_UK
dc.identifier.scopusid2-s2.0-84907222900en_UK
dc.identifier.wtid621081en_UK
dc.contributor.orcid0000-0002-7063-7491en_UK
dc.date.accepted2014-06-27en_UK
dcterms.dateAccepted2014-06-27en_UK
dc.date.filedepositdate2015-10-02en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorSilva, Sandro P|en_UK
local.rioxx.authorDilcher, Meik|en_UK
local.rioxx.authorWeber, Franziska|en_UK
local.rioxx.authorHufert, Frank T|en_UK
local.rioxx.authorWeidmann, Manfred|0000-0002-7063-7491en_UK
local.rioxx.authorCardoso, Jedson F|en_UK
local.rioxx.authorCarvalho, Valeria L|en_UK
local.rioxx.authorChang, Jannifer O|en_UK
local.rioxx.authorMartins, Livia C|en_UK
local.rioxx.authorLima, Clayton P S|en_UK
local.rioxx.authorSilva, Daisy E A|en_UK
local.rioxx.authorVianez-Junior, Joao L S G|en_UK
local.rioxx.authorPopov, Vsevolod L|en_UK
local.rioxx.authorTravassos da Rosa, Amelia P A|en_UK
local.rioxx.authorTesh, Robert B|en_UK
local.rioxx.authorVasconcelos, Pedro F C|en_UK
local.rioxx.authorNunes, Marcio R T|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate3000-01-01en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameSilva et al_JGV_2014.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0022-1317en_UK
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