Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/22066
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dc.contributor.authorBetancor, Monicaen_UK
dc.contributor.authorSprague, Matthewen_UK
dc.contributor.authorSayanova, Olgaen_UK
dc.contributor.authorUsher, Sarahen_UK
dc.contributor.authorCampbell, Patricken_UK
dc.contributor.authorNapier, Johnathan Aen_UK
dc.contributor.authorCaballero, Maria Joseen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.date.accessioned2015-10-09T00:07:28Z-
dc.date.available2015-10-09T00:07:28Z-
dc.date.issued2015-07-01en_UK
dc.identifier.urihttp://hdl.handle.net/1893/22066-
dc.description.abstractCurrently, one alternative for dietary fish oil (FO) in aquafeeds is vegetable oils (VO) that are devoid of omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFAs). Entirely new sources of n-3 LC-PUFA such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids through de novo production are a potential solution to fill the gap between supply and demand of these important nutrients. Camelina sativa was metabolically engineered to produce a seed oil (ECO) with N20% EPA and its potential to substitute for FO in Atlantic salmon feeds was tested. Fish were fed with one of the three experimental diets containing FO, wild-type camelina oil (WCO) or ECO as the sole lipid sources for 7 weeks. Inclusion of ECO did not affect any of the performance parameters studied and enhanced apparent digestibility of individual n-6 and n-3 PUFA compared to dietaryWCO. High levels of EPA were maintained in brain, liver and intestine (pyloric caeca), and levels of DPA and DHA were increased in liver and intestine of fish fed ECO compared to fish fed WCO likely due to increased LC-PUFA biosynthesis based on up-regulation of the genes. Fish fed ECO showed slight lipid accumulation within hepatocytes similar to that with WCO, although not significantly different to fish fed FO. The regulation of a small number of genes could be attributed to the specific effect of ECO (311 features) with metabolismbeing the most affected category. The EPA oil from transgenic Camelina (ECO) could be used as a substitute for FO, however it is a hybrid oil containing both FO (EPA) and VO (18:2n-6) fatty acid signatures that resulted in similarly mixed metabolic and physiological responses.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationBetancor M, Sprague M, Sayanova O, Usher S, Campbell P, Napier JA, Caballero MJ & Tocher DR (2015) Evaluation of a high-EPA oil from transgenic Camelina sativa in feeds for Atlantic salmon (Salmo salar L.): Effects on tissue fatty acid composition, histology and gene expression. Aquaculture, 444, pp. 1-12. https://doi.org/10.1016/j.aquaculture.2015.03.020en_UK
dc.rightsThis article is open-access. Open access publishing allows free access to and distribution of published articles where the author retains copyright of their work by employing a Creative Commons attribution licence. Proper attribution of authorship and correct citation details should be given.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_UK
dc.subjectEPAen_UK
dc.subjectCamelinaen_UK
dc.subjectAquacultureen_UK
dc.subjectFish oilen_UK
dc.subjectPyloric caecaen_UK
dc.subjectmicroarrayen_UK
dc.titleEvaluation of a high-EPA oil from transgenic Camelina sativa in feeds for Atlantic salmon (Salmo salar L.): Effects on tissue fatty acid composition, histology and gene expressionen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1016/j.aquaculture.2015.03.020en_UK
dc.identifier.pmid26146421en_UK
dc.citation.jtitleAquacultureen_UK
dc.citation.issn0044-8486en_UK
dc.citation.volume444en_UK
dc.citation.spage1en_UK
dc.citation.epage12en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.funderBiomar Ltden_UK
dc.contributor.funderBiotechnology and Biological Sciences Research Councilen_UK
dc.contributor.funderBiomar Ltden_UK
dc.author.emaildrt1@stir.ac.uken_UK
dc.citation.date26/03/2015en_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationRothamsted Researchen_UK
dc.contributor.affiliationRothamsted Researchen_UK
dc.contributor.affiliationBioMar U.K.en_UK
dc.contributor.affiliationRothamsted Researchen_UK
dc.contributor.affiliationUniversidad de Las Palmas de Gran Canariaen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000353644800001en_UK
dc.identifier.scopusid2-s2.0-84946240353en_UK
dc.identifier.wtid594393en_UK
dc.contributor.orcid0000-0003-1626-7458en_UK
dc.contributor.orcid0000-0002-0723-2387en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dc.date.accepted2015-03-19en_UK
dc.date.filedepositdate2015-07-24en_UK
dc.relation.funderprojectEvaluating novel plant oilseeds enriched in omega-3 long-chain polyunsaturated fatty acids to support sustainable development of aquacultureen_UK
dc.relation.funderprojectEvaluating novel plant oilseeds enriched in omega-3 long-chain polyunsaturated fatty acids to support sustainable development of aquacultureen_UK
dc.relation.funderrefBB/J001252/1en_UK
dc.relation.funderrefBB/J001252/1en_UK
Appears in Collections:Aquaculture Journal Articles

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