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dc.contributor.authorBetancor, Monica-
dc.contributor.authorAlmaida, Pedro-
dc.contributor.authorSprague, Matthew-
dc.contributor.authorHernandez, A-
dc.contributor.authorTocher, Douglas R-
dc.description.abstractMitochondrial decay is generally associated with impairment in the organelle bioenergetics function and increased oxidative stress, and it appears that deterioration of mitochondrial inner membrane phospholipids (PL) and accumulation of mitochondrial DNA (mtDNA) mutations are among the main mechanisms involved in this process. In the present study, mitochondrial membrane PL compositions, oxidative status (TBARS content and SOD activity) and mtDNA gene expression of muscle and liver were analyzed in zebrafish fed two diets with lipid supplied either by rapeseed oil (RO) or a blend 60:40 of RO and DHA500 TG oil (DHA). Two feeding trials were performed using zebrafish from the same population of two ages (8- and 21-months). Dietary FA composition affected fish growth in 8-month-old animals, which could be related with an increase in stress promoted by diet composition. Lipid peroxidation was considerably higher in mitochondria of 8-month-old zebrafish fed the DHA diet than in animals fed the RO diet. This could indicate higher oxidative damage to mitochondrial lipids, very likely due to increased incorporation of DHA in PL of mitochondrial membranes. Lipids would be among the first molecules affected by mitochondrial reactive oxygen species, and lipid peroxidation could propagate oxidative reactions that would damage other molecules, including mtDNA. Mitochondrial lipid peroxidation and gene expression of 21-month-old fish showed lower responsiveness to diet composition than those of younger fish. Differences found in the effect of diet composition on mitochondrial lipids between the two age groups could be indicating age-related changes in the ability to maintain structural homeostasis of mitochondrial membranes.en_UK
dc.relationBetancor M, Almaida P, Sprague M, Hernandez A & Tocher DR (2015) Roles of selenoprotein antioxidant protection in zebrafish, Danio rerio, subjected to dietary oxidative stress, Fish Physiology and Biochemistry, 41 (3), pp. 705-720.-
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dc.subjectfatty aciden_UK
dc.subjectoxidative stressen_UK
dc.titleRoles of selenoprotein antioxidant protection in zebrafish, Danio rerio, subjected to dietary oxidative stressen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreasonPublisher requires embargo of 12 months after formal publication.-
dc.citation.jtitleFish Physiology and Biochemistry-
dc.type.statusPost-print (author final draft post-refereeing)-
dc.contributor.affiliationUniversity of Stirling-
Appears in Collections:Aquaculture Journal Articles

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