Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/21924
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dc.contributor.authorEl Wahed, Ahmed Abden_UK
dc.contributor.authorPatel, Pranaven_UK
dc.contributor.authorFaye, Oumaren_UK
dc.contributor.authorThaloengsok, Sasikanyaen_UK
dc.contributor.authorHeidenreich, Dorisen_UK
dc.contributor.authorMatangkasombut, Ponpanen_UK
dc.contributor.authorManopwisedjaroen, Khajohnpongen_UK
dc.contributor.authorSakuntabhai, Anavajen_UK
dc.contributor.authorSall, Amadou Aen_UK
dc.contributor.authorHufert, Frank Ten_UK
dc.contributor.authorWeidmann, Manfreden_UK
dc.date.accessioned2015-07-17T23:35:21Z-
dc.date.available2015-07-17T23:35:21Z-
dc.date.issued2015-06-15en_UK
dc.identifier.othere0129682en_UK
dc.identifier.urihttp://hdl.handle.net/1893/21924-
dc.description.abstractBackground: Over 2.5 billion people are exposed to the risk of contracting dengue fever (DF). Early diagnosis of DF helps to diminish its burden on public health. Real-time reverse transcription polymerase amplification assays (RT-PCR) are the standard method for molecular detection of the dengue virus (DENV). Real-time RT-PCR analysis is not suitable for on-site screening since mobile devices are large, expensive, and complex. In this study, two RT-recombinase polymerase amplification (RT-RPA) assays were developed to detect DENV1-4. Methodology/Principal Findings: Using two quantitative RNA molecular standards, the analytical sensitivity of a RT-RPA targeting the 3´non-translated region of DENV1-4 was found to range from 14 (DENV4) to 241 (DENV1-3) RNA molecules detected. The assay was specific and did not cross detect other Flaviviruses. The RT-RPA assay was tested in a mobile laboratory combining magnetic-bead based total nucleic acid extraction and a portable detection device in Kedougou (Senegal) and in Bangkok (Thailand). In Kedougou, the RT-RPA was operated at an ambient temperature of 38°C with auxiliary electricity tapped from a motor vehicle and yielded a clinical sensitivity and specificity of 98% (n=31) and 100% (n=23), respectively. While in the field trial in Bangkok, the clinical sensitivity and specificity were 72% (n=90) and 100%(n=41), respectively. Conclusions/Significance: During the first 5 days of infection, the developed DENV1-4 RT-RPA assays constitute a suitable accurate and rapid assay for DENV diagnosis. Moreover, the use of a portable fluorescence-reading device broadens its application potential to the point-of-care for outbreak investigations.en_UK
dc.language.isoenen_UK
dc.publisherPublic Library of Scienceen_UK
dc.relationEl Wahed AA, Patel P, Faye O, Thaloengsok S, Heidenreich D, Matangkasombut P, Manopwisedjaroen K, Sakuntabhai A, Sall AA, Hufert FT & Weidmann M (2015) Recombinase Polymerase Amplification Assay for Rapid Diagnostics of Dengue Infection. PLoS ONE, 10 (6), Art. No.: e0129682. https://doi.org/10.1371/journal.pone.0129682en_UK
dc.rights© 2015 Abd El Wahed et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_UK
dc.titleRecombinase Polymerase Amplification Assay for Rapid Diagnostics of Dengue Infectionen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1371/journal.pone.0129682en_UK
dc.citation.jtitlePLoS ONEen_UK
dc.citation.issn1932-6203en_UK
dc.citation.volume10en_UK
dc.citation.issue6en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailm.w.weidmann@stir.ac.uken_UK
dc.contributor.affiliationGerman Primate Centeren_UK
dc.contributor.affiliationRobert Koch Instituteen_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationMahidol Universityen_UK
dc.contributor.affiliationGeorg-August University Gottingenen_UK
dc.contributor.affiliationMahidol Universityen_UK
dc.contributor.affiliationMahidol Universityen_UK
dc.contributor.affiliationInstitut Pasteuren_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationBrandenburg Medical School Theodor-Fontaneen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000356329900091en_UK
dc.identifier.scopusid2-s2.0-84937013070en_UK
dc.identifier.wtid595968en_UK
dc.contributor.orcid0000-0002-7063-7491en_UK
dc.date.accepted2015-05-12en_UK
dcterms.dateAccepted2015-05-12en_UK
dc.date.filedepositdate2015-06-22en_UK
rioxxterms.apcpaiden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorEl Wahed, Ahmed Abd|en_UK
local.rioxx.authorPatel, Pranav|en_UK
local.rioxx.authorFaye, Oumar|en_UK
local.rioxx.authorThaloengsok, Sasikanya|en_UK
local.rioxx.authorHeidenreich, Doris|en_UK
local.rioxx.authorMatangkasombut, Ponpan|en_UK
local.rioxx.authorManopwisedjaroen, Khajohnpong|en_UK
local.rioxx.authorSakuntabhai, Anavaj|en_UK
local.rioxx.authorSall, Amadou A|en_UK
local.rioxx.authorHufert, Frank T|en_UK
local.rioxx.authorWeidmann, Manfred|0000-0002-7063-7491en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2015-06-22en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by/4.0/|2015-06-22|en_UK
local.rioxx.filenameWeidmann_Plos One_2015.pdfen_UK
local.rioxx.filecount1en_UK
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