Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/21920
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dc.contributor.authorNally, Jarlath Een_UK
dc.contributor.authorMullen, Williamen_UK
dc.contributor.authorCallanan, John Jen_UK
dc.contributor.authorMischak, Haralden_UK
dc.contributor.authorAlbalat, Amayaen_UK
dc.date.accessioned2015-07-10T23:16:05Z-
dc.date.available2015-07-10T23:16:05Z-
dc.date.issued2015-06en_UK
dc.identifier.urihttp://hdl.handle.net/1893/21920-
dc.description.abstractPurpose: Pathogenic leptospires colonize the renal tubules of reservoir hosts of infection and are excreted via urine into the environment. Asymptomatic reservoir hosts include a wide range of domestic and wild animal species and include cattle, dogs, and rats that can persistently excrete large numbers of pathogenic leptospires over many months. A similar presentation has been observed in humans categorized as "long-term asymptomatic individuals" as they excreted leptospires in the absence of any clinical symptoms or positive serology. Experimental design: In the current study, the urine of experimentally infected rats, which showed no clinical signs or positive serology, was analyzed by CE-MS to identify urinary biomarkers of chronic infection. Results: A discriminating peptide pattern of 43 polypeptides provided a sensitivity of 93%, a specificity of 83%, and an accuracy of 90% for the identification of urine from chronically infected rats (p < 0.05, AUC > 90%). The majority of discriminating peptides were decreased in abundance in urine of chronically infected rats, including a peptide derived from neprilysin, a membrane metalloendopeptidase, the expression of which has previously been shown to be diminished in infected urine. Conclusion and clinical relevance: Results highlight the diagnostic capabilities of urinary biomarkers to identify reservoir hosts of leptospirosis using CE coupled to MS.en_UK
dc.language.isoenen_UK
dc.publisherWiley-Blackwellen_UK
dc.relationNally JE, Mullen W, Callanan JJ, Mischak H & Albalat A (2015) Detection of urinary biomarkers in reservoir hosts of leptospirosis by capillary electrophoresis-mass spectrometry. PROTEOMICS - Clinical Applications, 9 (5-6), pp. 543-551. https://doi.org/10.1002/prca.201400205en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectBiomarkeren_UK
dc.subjectCapillary electrophoresis-mass spectrometryen_UK
dc.subjectLeptospiraen_UK
dc.subjectLeptospirosisen_UK
dc.subjectUrineen_UK
dc.titleDetection of urinary biomarkers in reservoir hosts of leptospirosis by capillary electrophoresis-mass spectrometryen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-18en_UK
dc.rights.embargoreason[Nally_et_al-2015-PROTEOMICS_-_Clinical_Applications.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1002/prca.201400205en_UK
dc.citation.jtitlePROTEOMICS - Clinical Applicationsen_UK
dc.citation.issn1862-8354en_UK
dc.citation.issn1862-8346en_UK
dc.citation.volume9en_UK
dc.citation.issue5-6en_UK
dc.citation.spage543en_UK
dc.citation.epage551en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailamaya.albalat@stir.ac.uken_UK
dc.citation.date17/04/2015en_UK
dc.contributor.affiliationUniversity College Dublin (UCD)en_UK
dc.contributor.affiliationUniversity of Glasgowen_UK
dc.contributor.affiliationRoss University School of Medicineen_UK
dc.contributor.affiliationUniversity of Glasgowen_UK
dc.contributor.affiliationComplex Systems - LEGACYen_UK
dc.identifier.isiWOS:000356351600012en_UK
dc.identifier.scopusid2-s2.0-84928138676en_UK
dc.identifier.wtid597643en_UK
dc.contributor.orcid0000-0002-8606-2995en_UK
dc.date.accepted2015-02-26en_UK
dcterms.dateAccepted2015-02-26en_UK
dc.date.filedepositdate2015-06-19en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorNally, Jarlath E|en_UK
local.rioxx.authorMullen, William|en_UK
local.rioxx.authorCallanan, John J|en_UK
local.rioxx.authorMischak, Harald|en_UK
local.rioxx.authorAlbalat, Amaya|0000-0002-8606-2995en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2999-12-18en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameNally_et_al-2015-PROTEOMICS_-_Clinical_Applications.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1862-8346en_UK
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