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dc.contributor.authorPalaiokostas, Christosen_UK
dc.contributor.authorBekaert, Michaëlen_UK
dc.contributor.authorKhan, Mohd Golam Quaderen_UK
dc.contributor.authorTaggart, Johnen_UK
dc.contributor.authorGharbi, Karimen_UK
dc.contributor.authorMcAndrew, Brendanen_UK
dc.contributor.authorPenman, Daviden_UK
dc.description.abstractBackground: Fish species often exhibit significant sexual dimorphism for commercially important traits. Accordingly, the control of phenotypic sex, and in particular the production of monosex cultures, is of particular interest to the aquaculture industry. Sex determination in the widely farmed Nile tilapia (Oreochromis niloticus) is complex, involving genomic regions on at least three chromosomes (chromosomes 1, 3 and 23) and interacting in certain cases with elevated early rearing temperature as well. Thus, sex ratios may vary substantially from 50%. Results: This study focused on mapping sex-determining quantitative trait loci (QTL) in families with skewed sex ratios. These included four families that showed an excess of males (male ratio varied between 64% and 93%) when reared at standard temperature (28°C) and a fifth family in which an excess of males (96%) was observed when fry were reared at 36°C for ten days from first feeding. All the samples used in the current study were genotyped for two single-nucleotide polymorphisms (rs397507167 and rs397507165) located in the expected major sex-determining region in linkage group 1 (LG 1). The only misassigned individuals were phenotypic males with the expected female genotype, suggesting that those offspring had undergone sex-reversal with respect to the major sex-determining locus. We mapped SNPs identified from double digest Restriction-site Associated DNA (ddRAD) sequencing in these five families. Three genetic maps were constructed consisting of 641, 175 and 1,155 SNPs from the three largest families. QTL analyses provided evidence for a novel genome-wide significant QTL in LG 20. Evidence was also found for another sex-determining QTL in the fifth family, in the proximal region of LG 1. Conclusions: Overall, the results from this study suggest that these previously undetected QTLs are involved in sex determination in the Nile tilapia, causing sex reversal (masculinisation) with respect to the XX genotype at the major sex-determining locus in LG 1.en_UK
dc.publisherBioMed Centralen_UK
dc.relationPalaiokostas C, Bekaert M, Khan MGQ, Taggart J, Gharbi K, McAndrew B & Penman D (2015) A novel sex-determining QTL in Nile tilapia (Oreochromis niloticus). BMC Genomics, 16 (1), Art. No.: 171.
dc.rights© 2015 Palaiokostas et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.en_UK
dc.subjectOreochromis niloticusen_UK
dc.subjectSex reversalen_UK
dc.subjectQTL mappingen_UK
dc.titleA novel sex-determining QTL in Nile tilapia (Oreochromis niloticus)en_UK
dc.typeJournal Articleen_UK
dc.citation.jtitleBMC Genomicsen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationBangladesh Agricultural Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Edinburghen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorPalaiokostas, Christos|en_UK
local.rioxx.authorBekaert, Michaël|0000-0002-1206-7654en_UK
local.rioxx.authorKhan, Mohd Golam Quader|en_UK
local.rioxx.authorTaggart, John|0000-0002-3843-9663en_UK
local.rioxx.authorGharbi, Karim|en_UK
local.rioxx.authorMcAndrew, Brendan|0000-0001-7384-5133en_UK
local.rioxx.authorPenman, David|0000-0001-8608-6631en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenamePalaiokostas et al_BMC Genomics_2015.pdfen_UK
Appears in Collections:Aquaculture Journal Articles

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