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dc.contributor.authorAlmaida, Pedroen_UK
dc.contributor.authorDe Santis, Christianen_UK
dc.contributor.authorRubio-Mejia, Olga Len_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.description.abstractMitochondria are among the first responders to various stressors that challenge the homeostasis of cells and organisms. Mitochondrial decay is generally associated with impairment in the organelle bioenergetics function and increased oxidative stress, and it appears that deterioration of mitochondrial inner membrane phospholipids (PL), particularly cardiolipin (CL), and accumulation of mitochondrial DNA (mtDNA) mutations are among the main mechanisms involved in this process. In the present study, liver mitochondrial membrane PL compositions, lipid peroxidation and mtDNA gene expression were analyzed in rainbow trout fed three diets with the same base formulation but with lipid supplied either by fish oil (FO), rapeseed oil (RO) or a high DHA oil (DHA) during six weeks. Specifically, two feeding trials were performed using fish from the same population of two ages (1 and 3 years), and PL class compositions of liver mitochondria, fatty acid composition of individual PL classes, TBARS content and mtDNA expression were determined. Dietary fatty acid composition strongly affected mitochondrial membrane composition from trout liver but observed changes did not fully reflect the diet, particularly when it contained high DHA. The changes were PL specific, CL being particularly resistant to changes in DHA. Some significant differences observed in expression of mtDNA with diet may suggest long-term dietary effects in mitochondrial gene expression which could affect electron transport chain function. All the changes were influenced by fish age, which could be related to the different growth rates observed between 1- and 3-year-old trout but that could also indicate age-related changes in the ability to maintain structural homeostasis of mitochondrial membranes.en_UK
dc.relationAlmaida P, De Santis C, Rubio-Mejia OL & Tocher DR (2015) Dietary fatty acids affect mitochondrial phospholipid compositions and mitochondrial gene expression of rainbow trout liver at different ages. Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, 185 (1), pp. 73-86.
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Publisher policy allows this work to be made available in this repository; The final publication is available at Springer via
dc.subjectfatty aciden_UK
dc.subjectrainbow trouten_UK
dc.subjectoxidative stressen_UK
dc.titleDietary fatty acids affect mitochondrial phospholipid compositions and mitochondrial gene expression of rainbow trout liver at different agesen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[Final accepted.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.citation.jtitleJournal of Comparative Physiology Ben_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorAlmaida, Pedro|en_UK
local.rioxx.authorDe Santis, Christian|en_UK
local.rioxx.authorRubio-Mejia, Olga L|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameFinal accepted.pdfen_UK
Appears in Collections:Aquaculture Journal Articles

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