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dc.contributor.authorWang, Shuqien_UK
dc.contributor.authorMonroig, Oscaren_UK
dc.contributor.authorTang, Guoxiaen_UK
dc.contributor.authorZhang, Liangen_UK
dc.contributor.authorYou, Cuihongen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorLi, Yuanyouen_UK
dc.description.abstractThe capacity for endogenous production of LC-PUFA from PUFA in euryhaline or diadromous fish is largely unknown other than for Atlantic salmon (Salmo salar), an anadromous species, which displays a freshwater pattern. The aim of the present study was to characterize the enzymes of the LC-PUFA pathway in Japanese eel (Anguilla japonica), the most important catadromous species currently being farmed. cDNAs of two key genes were cloned and functional assays showed they encoded a desaturase (Fads2) with D6 and D8 activity and an elongase (Elovl5) with activity towards C18 and C20 PUFA, with activities similar to marine fish and an D6/D8 activity ratio similar to Atlantic salmon. Furthermore, tissue distribution of the mRNA showed a clear marine pattern with highest expression in brain and eye. Phylogenetic analysis placed the eel cDNAs in line with classical taxonomy. The data suggest that diadromous species display a pattern of LC-PUFA biosynthesis capacity that likely reflects the environmental and nutritional influence of their early life stages rather than those of adult fish. Future studies aim to establish the full range of PUFA desaturases and elongases in Japanese eel and to provide further insight to the importance and relevance of LC-PUFA biosynthesis in fish species and the influence of diadromy.en_UK
dc.relationWang S, Monroig O, Tang G, Zhang L, You C, Tocher DR & Li Y (2014) Investigating long-chain polyunsaturated fatty acid biosynthesis in teleost fish: Functional characterization of fatty acyl desaturase (Fads2) and Elovl5 elongase in the catadromous species, Japanese eel Anguilla japonica. Aquaculture, 434, pp. 57-65.
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. this is the author’s version of a work that was accepted for publication in Aquaculture. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Aquaculture, Volume 434, 20 October 2014, Pages 57–65. doi:10.1016/j.aquaculture.2014.07.016en_UK
dc.subjectJapanese eelen_UK
dc.subjectLong-chain polyunsaturated fatty acidsen_UK
dc.titleInvestigating long-chain polyunsaturated fatty acid biosynthesis in teleost fish: Functional characterization of fatty acyl desaturase (Fads2) and Elovl5 elongase in the catadromous species, Japanese eel Anguilla japonicaen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[Eel Final.pdf] Publisher requires embargo of 24 months after formal publication.en_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.funderNational Natural Science Foundation of Chinaen_UK
dc.contributor.funderEuropean Community Framework Programmeen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationShantou Universityen_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorWang, Shuqi|en_UK
local.rioxx.authorMonroig, Oscar|0000-0001-8712-0440en_UK
local.rioxx.authorTang, Guoxia|en_UK
local.rioxx.authorZhang, Liang|en_UK
local.rioxx.authorYou, Cuihong|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorLi, Yuanyou|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameEel Final.pdfen_UK
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