Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/21419
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dc.contributor.authorPereiro, Patricia-
dc.contributor.authorDios, Sonia-
dc.contributor.authorBoltana, Sebastian-
dc.contributor.authorColl, Julio M-
dc.contributor.authorEstepa, Amparo-
dc.contributor.authorMacKenzie, Simon-
dc.contributor.authorNovoa, Beatriz-
dc.contributor.authorFigueras, Antonio-
dc.date.accessioned2015-07-23T11:06:34Z-
dc.date.available2015-07-23T11:06:34Z-
dc.date.issued2014-08-06-
dc.identifier.othere104509-
dc.identifier.urihttp://hdl.handle.net/1893/21419-
dc.description.abstractDNA vaccines encoding the viral G glycoprotein show the most successful protection capability against fish rhabdoviruses. Nowadays, the molecular mechanisms underlying the protective response remain still poorly understood. With the aim of shedding light on the protection conferred by the DNA vaccines based in the G glycoprotein of viral haemorrhagic septicaemia virus (VHSV) in turbot (Scophthalmus maximus) we have used a specific microarray highly enriched in antiviral sequences to carry out the transcriptomic study associated to VHSV DNA vaccination/infection. The differential gene expression pattern in response to empty plasmid (pMCV1.4) and DNA vaccine (pMCV1.4-G860) intramuscular administration with regard to non-stimulated turbot was analyzed in head kidney at 8, 24 and 72 hours post-vaccination. Moreover, the effect of VHSV infection one month after immunization was also analyzed in vaccinated and non-vaccinated fish at the same time points. Genes implicated in the Toll-like receptor signalling pathway, IFN inducible/regulatory proteins, numerous sequences implicated in apoptosis and cytotoxic pathways, MHC class I antigens, as well as complement and coagulation cascades among others were analyzed in the different experimental groups. Fish receiving the pMCV1.4-G860 vaccine showed transcriptomic patterns very different to the ones observed in pMCV1.4-injected turbot after 72 h. On the other hand, VHSV challenge in vaccinated and non-vaccinated turbot induced a highly different response at the transcriptome level, indicating a very relevant role of the acquired immunity in vaccinated fish able to alter the typical innate immune response profile observed in non-vaccinated individuals. This exhaustive transcriptome study will serve as a complete overview for a better understanding of the crosstalk between the innate and adaptive immune response in fish after viral infection/vaccination. Moreover, it provides interesting clues about molecules with a potential use as vaccine adjuvants, antiviral treatments or markers for vaccine efficiency monitoring.en_UK
dc.language.isoen-
dc.publisherPublic Library of Science-
dc.relationPereiro P, Dios S, Boltana S, Coll JM, Estepa A, MacKenzie S, Novoa B & Figueras A (2014) Transcriptome profiles associated to VHSV infection or DNA vaccination in turbot (Scophthalmus maximus), PLoS ONE, 9 (8), Art. No.: e104509.-
dc.rights© 2014 Pereiro et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.-
dc.titleTranscriptome profiles associated to VHSV infection or DNA vaccination in turbot (Scophthalmus maximus)en_UK
dc.typeJournal Articleen_UK
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0104509-
dc.citation.jtitlePLoS ONE-
dc.citation.issn1932-6203-
dc.citation.volume9-
dc.citation.issue8-
dc.citation.publicationstatusPublished-
dc.citation.peerreviewedRefereed-
dc.type.statusPublisher version (final published refereed version)-
dc.author.emailsimon.mackenzie@stir.ac.uk-
dc.contributor.affiliationSpanish National Research Council (CSIC)-
dc.contributor.affiliationSpanish National Research Council (CSIC)-
dc.contributor.affiliationAquaculture-
dc.contributor.affiliationINIA-SIGT–Biotecnologia-
dc.contributor.affiliationThe Miguel Hernandez University of Elche-
dc.contributor.affiliationComplex Systems-
dc.contributor.affiliationSpanish National Research Council (CSIC)-
dc.contributor.affiliationSpanish National Research Council (CSIC)-
dc.identifier.isi000339995100080-
Appears in Collections:Aquaculture Journal Articles

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