Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/21413
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dc.contributor.authorCrespo, Diegoen_UK
dc.contributor.authorBonnet, Emilieen_UK
dc.contributor.authorRoher, Nereaen_UK
dc.contributor.authorMacKenzie, Simonen_UK
dc.contributor.authorKrasnov, Alekseien_UK
dc.contributor.authorGoetz, Frederick Wen_UK
dc.contributor.authorBobe, Julienen_UK
dc.contributor.authorPlanas, Josep Ven_UK
dc.date.accessioned2015-01-28T23:34:08Z-
dc.date.available2015-01-28T23:34:08Z-
dc.date.issued2010-04-12en_UK
dc.identifier.other34en_UK
dc.identifier.urihttp://hdl.handle.net/1893/21413-
dc.description.abstractBackground: The relevance of immune-endocrine interactions to the regulation of ovarian function in teleosts is virtually unexplored. As part of the innate immune response during infection, a number of cytokines such as tumor necrosis factor alpha (TNF alpha) and other immune factors, are produced and act on the reproductive system. However, TNF alpha is also an important physiological player in the ovulatory process in mammals. In the present study, we have examined for the first time the effects of TNF alpha in vitro in preovulatory ovarian follicles of a teleost fish, the brown trout (Salmo trutta). Methods: To determine the in vivo regulation of TNF alpha expression in the ovary, preovulatory brook trout (Salvelinus fontinalis) were injected intraperitoneally with either saline or bacterial lipopolysaccharide (LPS). In control and recombinant trout TNF alpha (rtTNF alpha)-treated brown trout granulosa cells, we examined the percentage of apoptosis by flow cytometry analysis and cell viability by propidium iodide (PI) staining. Furthermore, we determined the in vitro effects of rtTNF alpha on follicle contraction and testosterone production in preovulatory brown trout ovarian follicles. In addition, we analyzed the gene expression profiles of control and rtTNF alpha-treated ovarian tissue by microarray and real-time PCR (qPCR) analyses. Results: LPS administration in vivo causes a significant induction of the ovarian expression of TNF alpha. Treatment with rtTNF alpha induces granulosa cell apoptosis, decreases granulosa cell viability and stimulates the expression of genes known to be involved in the normal ovulatory process in trout. In addition, rtTNF alpha causes a significant increase in follicle contraction and testosterone production. Also, using a salmonid-specific microarray platform (SFA2.0 immunochip) we observed that rtTNF alpha induces the expression of genes known to be involved in inflammation, proteolysis and tissue remodeling. Furthermore, the expression of kallikrein, TOP-2, serine protease 23 and ADAM 22, genes that have been postulated to be involved in proteolytic and tissue remodeling processes during ovulation in trout, increases in follicles incubated in the presence of rtTNF alpha. Conclusions: In view of these results, we propose that TNF alpha could have an important role in the biomechanics of follicle weakening, ovarian rupture and oocyte expulsion during ovulation in trout, primarily through its stimulation of follicular cell apoptosis and the expression of genes involved in follicle wall proteolysis and contraction.en_UK
dc.language.isoenen_UK
dc.publisherBioMed Centralen_UK
dc.relationCrespo D, Bonnet E, Roher N, MacKenzie S, Krasnov A, Goetz FW, Bobe J & Planas JV (2010) Cellular and molecular evidence for a role of tumor necrosis factor alpha in the ovulatory mechanism of trout. Reproductive Biology and Endocrinology, 8, Art. No.: 34. https://doi.org/10.1186/1477-7827-8-34en_UK
dc.rights© 2010 Crespo et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/en_UK
dc.titleCellular and molecular evidence for a role of tumor necrosis factor alpha in the ovulatory mechanism of trouten_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1186/1477-7827-8-34en_UK
dc.citation.jtitleReproductive Biology and Endocrinologyen_UK
dc.citation.issn1477-7827en_UK
dc.citation.volume8en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailsimon.mackenzie@stir.ac.uken_UK
dc.contributor.affiliationUniversitat de Barcelonaen_UK
dc.contributor.affiliationFrench National Institute for Agricultural Research (INRA)en_UK
dc.contributor.affiliationUniversitat Autonoma de Barcelonaen_UK
dc.contributor.affiliationComplex Systems - LEGACYen_UK
dc.contributor.affiliationNOFIMA ASen_UK
dc.contributor.affiliationUniversity of Wisconsin-Milwaukeeen_UK
dc.contributor.affiliationFrench National Institute for Agricultural Research (INRA)en_UK
dc.contributor.affiliationUniversitat de Barcelonaen_UK
dc.identifier.isiWOS:000278329000001en_UK
dc.identifier.scopusid2-s2.0-77950670671en_UK
dc.identifier.wtid605671en_UK
dc.contributor.orcid0000-0003-1845-6826en_UK
dcterms.dateAccepted2010-04-12en_UK
dc.date.filedepositdate2015-01-28en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorCrespo, Diego|en_UK
local.rioxx.authorBonnet, Emilie|en_UK
local.rioxx.authorRoher, Nerea|en_UK
local.rioxx.authorMacKenzie, Simon|0000-0003-1845-6826en_UK
local.rioxx.authorKrasnov, Aleksei|en_UK
local.rioxx.authorGoetz, Frederick W|en_UK
local.rioxx.authorBobe, Julien|en_UK
local.rioxx.authorPlanas, Josep V|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2015-01-28en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by/2.0/|2015-01-28|en_UK
local.rioxx.filenameMacKenzie_RBE 2010.pdfen_UK
local.rioxx.filecount1en_UK
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