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Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Identification of candidate antimicrobial peptides derived from abalone hemocyanin
Author(s): Zhuang, Jun
Coates, Christopher
Zhu, Hongtao
Zhu, Ping
Wu, Zujian
Xie, Lianhui
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Keywords: Haemocyanin
Antimicrobial peptide
Innate immunity
In silico
Issue Date: Mar-2015
Date Deposited: 12-Dec-2014
Citation: Zhuang J, Coates C, Zhu H, Zhu P, Wu Z & Xie L (2015) Identification of candidate antimicrobial peptides derived from abalone hemocyanin. Developmental and Comparative Immunology, 49 (1), pp. 96-102.
Abstract: Haemocyanins present in invertebrate hemolymph are multifunctional proteins, responsible for oxygen transport and contributing to innate immunity through phenoloxidase-like activity. In arthropods, haemocyanin has been identified as a source of broad-spectrum antimicrobial peptides during infection. Conversely, no haemocyanin-derived antimicrobial peptides have been reported for molluscs. The present study describes a putative antimicrobial region, termed haliotisin, located within the linking sequence between the α-helical domain and β-sheet domain of abalone (Haliotis tuberculata) haemocyanin functional unit E. A series of synthetic peptides based on overlapping fragments of the haliotisin region were tested for their bactericidal potential. Incubating Gram-positive and Gram-negative bacteria in the presence of certain haliotisin peptides, notably peptides 3-4-5 (DTFDYKKFGYRYDSLELEGRSISRIDELIQQRQEKDRTFAGFLLKGFGTSAS) led to reductions in microbial growth. Furthermore, transmission electron micrographs of haliotisin-treated bacteria revealed damages to the microbial cell wall. Data discussed here provides the first evidence to suggest that molluscan haemocyanin may act as a source of anti-infective peptides.
DOI Link: 10.1016/j.dci.2014.11.008
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