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Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Zoothamnium duplicatum infestation of cultured horseshoe crabs (Limulus polyphemus)
Author(s): Shinn, Andrew
Muhlholzl, Alexander P
Coates, Christopher
Freeman, Mark
Metochis, Christoforos
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Issue Date: Feb-2015
Date Deposited: 12-Dec-2014
Citation: Shinn A, Muhlholzl AP, Coates C, Freeman M & Metochis C (2015) Zoothamnium duplicatum infestation of cultured horseshoe crabs (Limulus polyphemus). Journal of Invertebrate Pathology, 125, pp. 81-86.
Abstract: An outbreak of the sessile peritrich Zoothamnium duplicatum in a pilot, commercial-scale Limulus polyphemus hatchery resulted in the loss of ∼ 96% (40,000) second/third instar larvae over a 61 day period. Peritich growth was heavy, leading to mechanical obstruction of the gills and physical damage. The peritrichs were controlled without resultant loss of juvenile crabs by administering 10 ppm chlorine in freshwater for 1 h and the addition of aquarium grade sand; a medium into which the crabs could burrow and facilitate cleaning of the carapace. Peritrich identity was confirmed from a partial SSU rDNA contiguous sequence of 1343 bp (99.7% similarity to Z. duplicatum).
DOI Link: 10.1016/j.jip.2014.12.002
Rights: Published in Journal of Invertebrate Pathology by Elsevier; Elsevier believes that individual authors should be able to distribute their AAMs for their personal voluntary needs and interests, e.g. posting to their websites or their institution’s repository, e-mailing to colleagues. However, our policies differ regarding the systematic aggregation or distribution of AAMs to ensure the sustainability of the journals to which AAMs are submitted. Therefore, deposit in, or posting to, subject-oriented or centralized repositories (such as PubMed Central), or institutional repositories with systematic posting mandates is permitted only under specific agreements between Elsevier and the repository, agency or institution, and only consistent with the publisher’s policies concerning such repositories. Voluntary posting of AAMs in the arXiv subject repository is permitted.

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