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Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Improved transfection of HUVEC and MEF cells using DNA complexes with magnetic nanoparticles in an oscillating field
Author(s): Lim, Jenson
Dobson, John
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Keywords: magnetic nanoparticles
mouse embryonic fibroblasts
human umbilical vein endothelial cells
oscillating magnet array
Issue Date: Aug-2012
Date Deposited: 16-Sep-2014
Citation: Lim J & Dobson J (2012) Improved transfection of HUVEC and MEF cells using DNA complexes with magnetic nanoparticles in an oscillating field. Journal of Genetics, 91 (2), pp. 223-227.
Abstract: First paragraph: Cells such as mouse embryonic fibroblasts (MEFs) and human umbilical vein endothelial cells (HUVECs) used in stem cell research and endothelial cell physiology and pathology studies are difficult to transfect using ‘standard' nonviral transfection methods. We have developed a novel gene delivery technique, which uses magnetic nanoparticles under the influence of an oscillating magnetic array. Here we report results from this technique showing significantly higher transfection efficiency in these cells compared to a static magnetic array and lipid reagents, with no effect on cell viabilities. Further in HUVECs, we report increasing gene transfection and expression with increasing oscillation frequencies and in serum-starved conditions.
DOI Link: 10.1007/s12041-012-0164-4
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