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dc.contributor.authorLim, Jenson-
dc.contributor.authorDupuy, Aurelien G-
dc.contributor.authorCritchley, David R-
dc.contributor.authorCaron, Emmanuelle-
dc.description.abstractThe small GTPase Rap1 and the cytoskeletal protein talin regulate binding of C3bi-opsonised red blood cells (RBC) to integrin α(M)β(2) in phagocytic cells, although the mechanism has not been investigated. Using COS-7 cells transfected with α(M)β(2), we show that Rap1 acts on the β(2) and not the α(M) chain, and that residues 732-761 of the β(2) subunit are essential for Rap1-induced RBC binding. Activation of α(M)β(2) by Rap1 was dependent on W747 and F754 in the β(2) tails, which are required for talin head binding, suggesting a link between Rap1 and talin in this process. Using talin1 knock-out cells or siRNA-mediated talin1 knockdown in the THP-1 monocytic cell line, we show that Rap1 acts upstream of talin but surprisingly, RIAM knockdown had little effect on integrin-mediated RBC binding or cell spreading. Interestingly, Rap1 and talin influence each other's localisation at phagocytic cups, and co-immunoprecipitation experiments suggest that they interact together. These results show that Rap1-mediated activation of α(M)β(2) in macrophages shares both common and distinct features from Rap1 activation of α(IIb)β(3) expressed in CHO cells.en_UK
dc.relationLim J, Dupuy AG, Critchley DR & Caron E (2010) Rap1 controls activation of the α(M)β(2) integrin in a talin-dependent manner, Journal of Cellular Biochemistry, 111 (4), pp. 999-1009.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.titleRap1 controls activation of the α(M)β(2) integrin in a talin-dependent manneren_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.citation.jtitleJournal of Cellular Biochemistry-
dc.type.statusPublisher version (final published refereed version)-
dc.contributor.affiliationBiological and Environmental Sciences-
dc.contributor.affiliationImperial College London-
dc.contributor.affiliationUniversity of Leicester-
dc.contributor.affiliationImperial College London-
Appears in Collections:Biological and Environmental Sciences Journal Articles

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