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dc.contributor.authorCougoule, Celineen_UK
dc.contributor.authorHoshino, Saikoen_UK
dc.contributor.authorDart, Annaen_UK
dc.contributor.authorLim, Jensonen_UK
dc.contributor.authorCaron, Emmanuelleen_UK
dc.description.abstractRho-family proteins play a central role in most actin-dependent processes, including the control and maintenance of cell shape, adhesion, motility, and phagocytosis. Activation of these GTP-binding proteins is tightly regulated spatially and temporally; however, very little is known of the mechanisms involved in their recruitment and activation in vivo. Because of its inducible, restricted signaling, phagocytosis offers an ideal physiological system to delineate the pathways linking surface receptors to actin remodeling via Rho GTPases. In this study, we investigated the involvement of early regulators of Fcgamma receptor signaling in Rac recruitment and activation. Using a combination of receptor mutagenesis, cellular, molecular, and pharmacological approaches, we show that Src family and Syk kinases control Rac and Vav function during phagocytosis. Importantly, both the immunoreceptor tyrosine-based activation motif within Fcgamma receptor cytoplasmic domain and Src kinase control the recruitment of Vav and Rac. However, Syk activity is dispensable for Vav and Rac recruitment. Moreover, we show that Rac and Cdc42 activities coordinate F-actin accumulation at nascent phagosomes. Our results provide new insights in the understanding of the spatiotemporal regulation of Rho-family GTPase function, and of Rac in particular, during phagocytosis. We believe they will contribute to a better understanding of more complex cellular processes, such as cell adhesion and migration.en_UK
dc.publisherAmerican Society for Biochemistry and Molecular Biologyen_UK
dc.relationCougoule C, Hoshino S, Dart A, Lim J & Caron E (2006) Dissociation of recruitment and activation of the small G-protein Rac during Fcgamma receptor-mediated phagocytosis. Journal of Biological Chemistry, 281 (13), pp. 8756-8764.
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.titleDissociation of recruitment and activation of the small G-protein Rac during Fcgamma receptor-mediated phagocytosisen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[2006 Cougoule et al Fc Rac phagocytosis.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.citation.jtitleJournal of Biological Chemistryen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationImperial College Londonen_UK
dc.contributor.affiliationImperial College Londonen_UK
dc.contributor.affiliationImperial College Londonen_UK
dc.contributor.affiliationBiological and Environmental Sciencesen_UK
dc.contributor.affiliationImperial College Londonen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorCougoule, Celine|en_UK
local.rioxx.authorHoshino, Saiko|en_UK
local.rioxx.authorDart, Anna|en_UK
local.rioxx.authorLim, Jenson|0000-0001-7417-356Xen_UK
local.rioxx.authorCaron, Emmanuelle|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filename2006 Cougoule et al Fc Rac phagocytosis.pdfen_UK
Appears in Collections:Biological and Environmental Sciences Journal Articles

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