Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/21007
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dc.contributor.authorAnthony, Nicola M-
dc.contributor.authorClifford, Stephen L-
dc.contributor.authorJohnson-Bawe, Mireille-
dc.contributor.authorAbernethy, Katharine-
dc.contributor.authorBruford, Michael W-
dc.contributor.authorWickings, E Jean-
dc.date.accessioned2014-09-04T00:37:06Z-
dc.date.issued2007-05-
dc.identifier.urihttp://hdl.handle.net/1893/21007-
dc.description.abstractNuclear integrations of mitochondrial DNA (Numts) are widespread in many taxa and if left undetected can confound phylogeny interpretation and bias estimates of mitochondrial DNA (mtDNA) diversity. This is particularly true in gorillas, where recent studies suggest multiple integrations of the first hypervariable (HV1) domain of the mitochondrial control region. Problems can also arise through the inadvertent incorporation of artifacts produced by in vitro recombination between sequence types during polymerase chain reaction amplification. This issue has attracted little attention yet could potentially exacerbate errors in databases already contaminated by Numts. Using a set of existing diagnostic tools, this study set out to systematically inventory Numts and PCR recombinants in a gorilla HV1 sequence database and address the degree to which existing public databases are contaminated. Phylogenetic analysis revealed three distinct gorilla HV1 Numt groups (I, II, and III) that could be readily differentiated from mtDNA sequences by Numt-specific diagnostic sites and sequence-based motifs. Several instances of genuine recombination were also identified by a suite of detection methods. The location of putative breakpoints was identified by eye and by likelihood analysis. Findings from this study reveal widespread nuclear contamination of gorilla HV1 GenBank databases and underline the importance of recognizing not only Numts but also PCR recombinant artifacts as potential sources of data contamination. Guidelines for the routine identification of Numts and in vitro recombinants are presented and should prove useful in the detection of similar artifacts in other species mtDNA databases.en_UK
dc.language.isoen-
dc.publisherElsevier-
dc.relationAnthony NM, Clifford SL, Johnson-Bawe M, Abernethy K, Bruford MW & Wickings EJ (2007) Distinguishing gorilla mitochondrial sequences from nuclear integrations and PCR recombinants: Guidelines for their diagnosis in complex sequence databases, Molecular Phylogenetics and Evolution, 43 (2), pp. 553-566.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.subjectNuclear integrationsen_UK
dc.subjectNumtsen_UK
dc.subjectMitochondrial DNAen_UK
dc.subjectHV1en_UK
dc.subjectGorillaen_UK
dc.subjectRecombinationen_UK
dc.subjectControl regionen_UK
dc.subjectPhylogeographyen_UK
dc.titleDistinguishing gorilla mitochondrial sequences from nuclear integrations and PCR recombinants: Guidelines for their diagnosis in complex sequence databasesen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31T00:00:00Z-
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.identifier.doihttp://dx.doi.org/10.1016/j.ympev.2006.09.013-
dc.citation.jtitleMolecular Phylogenetics and Evolution-
dc.citation.issn1055-7903-
dc.citation.volume43-
dc.citation.issue2-
dc.citation.spage553-
dc.citation.epage566-
dc.citation.publicationstatusPublished-
dc.citation.peerreviewedRefereed-
dc.type.statusPublisher version (final published refereed version)-
dc.author.emailk.a.abernethy@stir.ac.uk-
dc.contributor.affiliationCardiff University-
dc.contributor.affiliationCardiff University-
dc.contributor.affiliationCentre International de Recherches Médicales de Franceville-
dc.contributor.affiliationBiological and Environmental Sciences-
dc.contributor.affiliationCardiff University-
dc.contributor.affiliationCentre International de Recherches Médicales de Franceville-
dc.rights.embargoterms2999-12-31-
dc.rights.embargoliftdate2999-12-31-
dc.identifier.isi000246918800017-
Appears in Collections:Biological and Environmental Sciences Journal Articles

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