Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/21002
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dc.contributor.authorLopez Jimena, Benjaminen_UK
dc.contributor.authorGarcia-Rosado, Estheren_UK
dc.contributor.authorInfante, Carlosen_UK
dc.contributor.authorCano, Ireneen_UK
dc.contributor.authorManchado, Manuelen_UK
dc.contributor.authorCastro, Doloresen_UK
dc.contributor.authorBorrego, Juan Jen_UK
dc.contributor.authorAlonso, Maria del Carmenen_UK
dc.date.accessioned2014-09-04T00:25:48Z-
dc.date.available2014-09-04T00:25:48Zen_UK
dc.date.issued2010-04en_UK
dc.identifier.urihttp://hdl.handle.net/1893/21002-
dc.description.abstractA non-destructive procedure based on nested RT-PCR and dot-blot hybridization has been developed for the detection of asymptomatic IPNV-carrier fish. The pair of primers designed for RT-PCR amplified a 599-bp fragment of the pVP2 region within the polyprotein gene, resulting in the detection of IPNV genotype III.1. The use of a nested RT-PCR allowed the amplification of IPNV genotypes III.1 and I.2. In addition, a 191-bp probe was designed for hybridization studies used in combination with the nested RT-PCR. The application of the nested RT-PCR to analyse blood samples from asymptomatic redbanded seabream, Pagrus auriga, and common seabream, P. pagrus, specimens showed a 53.1% and 77.8% prevalence of IPNV-carriers, respectively. The combination of nested RT-PCR and dot-blot hybridization increased the detection rates up to 100% for redbanded seabream and 94.4% for common seabream. Therefore, the protocol described in this study is highly sensitive and specific for the detection of IPNV in asymptomatic carrier fish, and, in addition, the results demonstrate the carrier state in two newly cultured sparid species in southern Spain.en_UK
dc.language.isoenen_UK
dc.publisherWiley-Blackwellen_UK
dc.relationLopez Jimena B, Garcia-Rosado E, Infante C, Cano I, Manchado M, Castro D, Borrego JJ & Alonso MdC (2010) Detection of infectious pancreatic necrosis virus (IPNV) from asymptomatic redbanded seabream, Pagrus auriga Valenciennes, and common seabream, Pagrus pagrus (L.), using a non-destructive procedure. Journal of Fish Diseases, 33 (4), pp. 311-319. https://doi.org/10.1111/j.1365-2761.2009.01123.xen_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectsparid fishen_UK
dc.subjectinfectious pancreatic necrosis virusen_UK
dc.subjectcarrier stateen_UK
dc.subjectnon-destructive procedureen_UK
dc.subjecthybridizationen_UK
dc.subjectPCR methodsen_UK
dc.titleDetection of infectious pancreatic necrosis virus (IPNV) from asymptomatic redbanded seabream, Pagrus auriga Valenciennes, and common seabream, Pagrus pagrus (L.), using a non-destructive procedureen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31en_UK
dc.rights.embargoreason[Lopez-Jimena et al 2010 JFD.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1111/j.1365-2761.2009.01123.xen_UK
dc.citation.jtitleJournal of Fish Diseasesen_UK
dc.citation.issn1365-2761en_UK
dc.citation.issn0140-7775en_UK
dc.citation.volume33en_UK
dc.citation.issue4en_UK
dc.citation.spage311en_UK
dc.citation.epage319en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailbenjamin.lopez-jimena1@stir.ac.uken_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Malagaen_UK
dc.contributor.affiliationCentro IFAPA, Spainen_UK
dc.contributor.affiliationUniversity of Malagaen_UK
dc.contributor.affiliationCentro IFAPA, Spainen_UK
dc.contributor.affiliationUniversity of Malagaen_UK
dc.contributor.affiliationUniversity of Malagaen_UK
dc.contributor.affiliationUniversity of Malagaen_UK
dc.identifier.isiWOS:000275531600004en_UK
dc.identifier.scopusid2-s2.0-77953906506en_UK
dc.identifier.wtid621305en_UK
dc.contributor.orcid0000-0002-0141-8113en_UK
dcterms.dateAccepted2010-04-30en_UK
dc.date.filedepositdate2014-08-21en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorLopez Jimena, Benjamin|0000-0002-0141-8113en_UK
local.rioxx.authorGarcia-Rosado, Esther|en_UK
local.rioxx.authorInfante, Carlos|en_UK
local.rioxx.authorCano, Irene|en_UK
local.rioxx.authorManchado, Manuel|en_UK
local.rioxx.authorCastro, Dolores|en_UK
local.rioxx.authorBorrego, Juan J|en_UK
local.rioxx.authorAlonso, Maria del Carmen|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2999-12-31en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameLopez-Jimena et al 2010 JFD.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0140-7775en_UK
Appears in Collections:Aquaculture Journal Articles

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