Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/20985
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dc.contributor.authorLopez, Jimena Benjamin-
dc.contributor.authorCherif, Nadia-
dc.contributor.authorGarcia-Rosado, Esther-
dc.contributor.authorInfante, Carlos-
dc.contributor.authorCano, Irene-
dc.contributor.authorCastro, Dolores-
dc.contributor.authorHammami, Salah-
dc.contributor.authorBorrego, Juan J-
dc.contributor.authorAlonso, Maria del Carmen-
dc.date.accessioned2014-09-05T23:36:39Z-
dc.date.issued2010-10-
dc.identifier.urihttp://hdl.handle.net/1893/20985-
dc.description.abstractAims: To detect the possible coexistence of striped jack nervous necrosis virus (SJNNV) and red-spotted grouper nervous necrosis virus (RGNNV) genotypes in a single fish, a methodology based on the combination of PCR amplification and blot hybridization has been developed and applied in this study. Methods and Results: The degenerate primers designed for the PCR procedure target the T4 region within the capsid gene, resulting in the amplification of both genotypes. The subsequent hybridization of these amplification products with two different specific digoxigenin-labelled probes resulted in the identification of both genotypes separately. The application of the RT-PCR protocol to analyse blood samples from asymptomatic wild meagre (Argyrosomus regius) specimens has shown a 46·87% of viral nervous necrosis virus carriers. The combination of RT-PCR and blot hybridization increases the detection rate up to 90·62%, and, in addition, it has shown the coexistence of both genotypes in 18 out of the 32 specimens analysed (56·25%). Conclusions: This study reports the coexistence of betanodaviruses belonging to two different genotypes (SJNNV and RGNNV) in wild fish specimens. Significance and Impact of the Study: This is the first report demonstrating the presence of SJNNV and RGNNV genotypes in the same specimen. This study also demonstrates a carrier state in this fish species for the first time.en_UK
dc.language.isoen-
dc.publisherWiley-Blackwell-
dc.relationLopez Jimena B, Cherif N, Garcia-Rosado E, Infante C, Cano I, Castro D, Hammami S, Borrego JJ & Alonso MdC (2010) A combined RT-PCR and dot-blot hybridization method reveals the coexistence of SJNNV and RGNNV betanodavirus genotypes in wild meagre (Argyrosomus regius), Journal of Applied Microbiology, 109 (4), pp. 1361-1369.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.subjectbetanodavirusen_UK
dc.subjectdegenerate primersen_UK
dc.subjectdot-blot hybridizationen_UK
dc.subjectRGNNVen_UK
dc.subjectRT-PCRen_UK
dc.subjectSJNNVen_UK
dc.titleA combined RT-PCR and dot-blot hybridization method reveals the coexistence of SJNNV and RGNNV betanodavirus genotypes in wild meagre (Argyrosomus regius)en_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31T00:00:00Z-
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.identifier.doihttp://dx.doi.org/10.1111/j.1365-2672.2010.04759.x-
dc.citation.jtitleJournal of Applied Microbiology-
dc.citation.issn1364-5072-
dc.citation.volume109-
dc.citation.issue4-
dc.citation.spage1361-
dc.citation.epage1369-
dc.citation.publicationstatusPublished-
dc.citation.peerreviewedRefereed-
dc.type.statusPublisher version (final published refereed version)-
dc.author.emailbenjamin.lopez-jimena1@stir.ac.uk-
dc.contributor.affiliationAquaculture-
dc.contributor.affiliationInstitute of Veterinary Research Tunisia-
dc.contributor.affiliationUniversity of Malaga-
dc.contributor.affiliationCentro IFAPA, Spain-
dc.contributor.affiliationUniversity of Malaga-
dc.contributor.affiliationUniversity of Malaga-
dc.contributor.affiliationInstitute of Veterinary Research Tunisia-
dc.contributor.affiliationUniversity of Malaga-
dc.contributor.affiliationUniversity of Malaga-
dc.rights.embargoterms2999-12-31-
dc.rights.embargoliftdate2999-12-31-
dc.identifier.isi000281895200026-
Appears in Collections:Aquaculture Journal Articles

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