Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorStolte, Ellen H-
dc.contributor.authorNabuurs, Sander B-
dc.contributor.authorBury, Nicholas R-
dc.contributor.authorSturm, Armin-
dc.contributor.authorFlik, Gert-
dc.contributor.authorSavelkoul, Huub F J-
dc.contributor.authorVerburg-van, Kemenade B M Lidy-
dc.description.abstractThe stress hormone cortisol is deeply involved in immune regulation in all vertebrates. Common carp (Cyprinus carpio L.) express four corticoid receptors that may modulate immune responses: three glucocorticoid receptors (GR); GR1, with two splice variants (GR1a and GR1b), GR2 and a single mineralocorticoid receptor (MR). All receptors are expressed as of 4 days post-fertilization and may thus play a critical role in development and functioning of the adult immune system. Immune tissues and cells predominantly express mRNA for GRs compared to mRNA for the MR. Three-dimensional protein structure modeling predicts, and transfection assays confirm that alternative splicing of GR1 does not influence the capacity to induce transcription of effector genes. When tested for cortisol activation, GR2 is the most sensitive corticoid receptor in carp, followed by the MR and GR1a and GR1b. Lipopolysacharide (LPS) treatment of head kidney phagocytes quickly induces GR1 expression and inhibits GR2 expression. Cortisol treatment in vivo enhances GR1a and MR mRNA expression, but only mildly, and cortisol treatment in vitro does not affect receptor expression of phagocytes. Cortisol has no direct effect on the LPS-induced receptor profile. Therefore, an immune rather than a stress stimulus regulates GR expression. Cortisol administered at stress levels to phagocytes in vitro significantly inhibits LPS-induced expression of the pro-inflammatory cytokines tumor necrosis factor alpha (TNF-α) and interleukin-12 (IL-12) (subunit p35) and of inducible nitric oxide synthase (iNOS) expression. A physiologically differential function for GR1 and GR2 in the immune response of fish to infection is indicated.en_UK
dc.relationStolte EH, Nabuurs SB, Bury NR, Sturm A, Flik G, Savelkoul HFJ & Verburg-van Kemenade BML (2008) Stress and innate immunity in carp: Corticosteroid receptors and pro-inflammatory cytokines, Molecular Immunology, 46 (1), pp. 70-79.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.subjectAlternative splicingen_UK
dc.subjectImmune systemen_UK
dc.subjectCytokine expressionen_UK
dc.subjectCommon carpen_UK
dc.titleStress and innate immunity in carp: Corticosteroid receptors and pro-inflammatory cytokinesen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.citation.jtitleMolecular Immunology-
dc.type.statusPublisher version (final published refereed version)-
dc.contributor.affiliationWageningen University-
dc.contributor.affiliationRadboud University Medical Centre Nijmegen-
dc.contributor.affiliationKing's College London-
dc.contributor.affiliationRadboud University Nijmegen-
dc.contributor.affiliationWageningen University-
dc.contributor.affiliationWageningen University-
Appears in Collections:Aquaculture Journal Articles

Files in This Item:
File Description SizeFormat 
stolte2008MI.pdf898.64 kBAdobe PDFUnder Permanent Embargo    Request a copy

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.