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dc.contributor.authorHerlin, Marine-
dc.contributor.authorTaggart, John-
dc.contributor.authorMcAndrew, Brendan-
dc.contributor.authorPenman, David-
dc.description.abstractParentage assignment is becoming increasingly popular as a means of monitoring the effects of aquaculture husbandry practices. As the complexity of such studies increase, likelihood-based methods for assignment are coming to the fore since, theoretically at least, they offer the possibility of accurate assignment with less screening overheads (fewer loci, less cost) compared to exclusion-based methods. We were able to explore this assertion through the analysis of the parental contribution to 300 Atlantic cod (Gadus morhua) fiy produced from a commercial mass spawning tank (containing 99 broodstock) on a single day. Five polymorphic DNA microsatellite loci were screened and three different datasets generated: 'error prone' raw genotypic data from automated allele calling; manually corrected data; and corrected data from a reduced number of loci (four). Parentage analysis was performed by three software packages-PAPA, CERVUS and FAP-which all have been used in aquaculture contexts. PAPA and CERVUS perform likelihood-based analyses, while FAP is exclusion-based. Striking differences in the allocation performance were noted both among programs and among datasets. With no allowance for error all three programs flagged up potential problems within datasets, particularly the error prone 'raw' genotypes. When allowance for a presumed low level of error was invoked using PAPA (uniform error rate = 0.0001-0.02) parentage was assigned for virtually all offspring, irrespective of the dataset used. Despite simulations suggesting that these assignments were likely to be accurate (correctness values >95%) substantial numbers of assignments (up to 18%) differed among the datasets. FAP gave more conservative (maximum of c. 78% of assignments resolved) and consistent assignments. The power of assignment declined significantly when only four loci were considered. CERVUS performed relatively poorly in terms of numbers of assignments made. Where comparisons were possible most of the identified parental-pairs agreed with FAP allocations. Reliance on likelihood-based assignments alone to resolve parentage in complex mass spawning tanks may be inappropriate. Furthermore, reducing the number of loci to a minimal set (based on likelihood predictions) could be counterproductive. Allocation methods should be more thoroughly assessed and fully described. (c) 2007 Elsevier B.V. All rights reserveden_UK
dc.relationHerlin M, Taggart J, McAndrew B & Penman D (2007) Parentage allocation in a complex situation: A large commercial Atlantic cod (Gadus morhua) mass spawning tank, Aquaculture, 272 (Supplement 1), pp. S195-S203.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.subjectAtlantic coden_UK
dc.subjectGadus morhuaen_UK
dc.subjectParental analysisen_UK
dc.subjectGenotyping erroren_UK
dc.titleParentage allocation in a complex situation: A large commercial Atlantic cod (Gadus morhua) mass spawning tanken_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.citation.issueSupplement 1-
dc.type.statusPublisher version (final published refereed version)-
dc.contributor.affiliationUniversity of Stirling-
Appears in Collections:Aquaculture Journal Articles

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