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dc.contributor.authorReis, D Ben_UK
dc.contributor.authorAcosta, Nieves Guadalupeen_UK
dc.contributor.authorAlmansa, Eduardoen_UK
dc.contributor.authorNavarro, Juan Carlosen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorMonroig, Oscaren_UK
dc.contributor.authorAndrade, Jose Pedroen_UK
dc.contributor.authorSykes, Antonio Ven_UK
dc.contributor.authorRodriguez, Covadongaen_UK
dc.description.abstractThe high mortalities observed during Octopus vulgaris paralarvae culture have been associated with a nutritional imbalance, with long-chain polyunsaturated fatty acids (LC-PUFA) appearing to have a critical role. In order to determine the in vivo capability of O. vulgaris hatchlings to incorporate and metabolise unsaturated fatty acids (FA), hatchlings were incubated in flat-bottom 6-well tissue culture plates at a density of 90 hatchlings/well in 10 mL of seawater (36‰). Incubations were performed with gentle stirring at 21 °C for 6 h with 0.2 μCi (0.3 μM) of [1−14C]-labelled FA including 18:1n−9, 18:2n−6, 18:3n−3, 20:4n−6 (ARA), 20:5n−3 (EPA) or 22:6n−3 (DHA), which were added directly to the seawater as their potassium salts bound to bovine serum al- bumin (BSA). A control treatment without [1−14C]FA was also assessed. O. vulgaris hatchlings not only possessed the ability to incorporate FA bound to BSA, but also to esterify them into phospholipid, with marked specificity. [1−14C]DHA and [1−14C]C18 FA substrates were mainly esterified into phosphatidylcholine, while [1−14C]ARA and [1−14C]EPA were esterified into phosphatidylethanolamine. The majority of radioactivity from [1−14C]FA incorporated into hatchling total lipid was recovered as unmodified FA with elongation being the only metabo- lism detected. Of the FA investigated, [1−14C]ARA was the most efficiently incorporated into hatchling lipids, but it was also the least modified FA. The fact that no desaturation activity was recorded towards the FA tested in this experiment may indicate that the nutritional requirements of O. vulgaris hatchlings in terms of FA are highly specific and LC-PUFA must be considered essential dietary nutrients.en_UK
dc.relationReis DB, Acosta NG, Almansa E, Navarro JC, Tocher DR, Monroig O, Andrade JP, Sykes AV & Rodriguez C (2014) In vivo metabolism of unsaturated fatty acids in Octopus vulgaris hatchlings determined by incubation with 14C-labelled fatty acids added directly to seawater as protein complexes. Aquaculture, 431, pp. 28-33.
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.subjectCommon octopusen_UK
dc.subjectLipid metabolismen_UK
dc.subjectOctopus vulgarisen_UK
dc.subjectRadiolabeled substratesen_UK
dc.subjectUnsaturated fatty acidsen_UK
dc.titleIn vivo metabolism of unsaturated fatty acids in Octopus vulgaris hatchlings determined by incubation with 14C-labelled fatty acids added directly to seawater as protein complexesen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[Pulpo 14C-metabolism (proof).pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationUniversity of Algarveen_UK
dc.contributor.affiliationUniversity of La Lagunaen_UK
dc.contributor.affiliationSpanish Institute of Oceanography (IEO)en_UK
dc.contributor.affiliationInstitute of Aquaculture Torre de la Salen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationComplex Systems - LEGACYen_UK
dc.contributor.affiliationUniversity of Algarveen_UK
dc.contributor.affiliationUniversity of Algarveen_UK
dc.contributor.affiliationUniversity of La Lagunaen_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorReis, D B|en_UK
local.rioxx.authorAcosta, Nieves Guadalupe|en_UK
local.rioxx.authorAlmansa, Eduardo|en_UK
local.rioxx.authorNavarro, Juan Carlos|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorMonroig, Oscar|0000-0001-8712-0440en_UK
local.rioxx.authorAndrade, Jose Pedro|en_UK
local.rioxx.authorSykes, Antonio V|en_UK
local.rioxx.authorRodriguez, Covadonga|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenamePulpo 14C-metabolism (proof).pdfen_UK
Appears in Collections:Aquaculture Journal Articles

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