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dc.contributor.authorEscadafal, Camilleen_UK
dc.contributor.authorFaye, Oumaren_UK
dc.contributor.authorSall, Amadou Aen_UK
dc.contributor.authorFaye, Ousmaneen_UK
dc.contributor.authorWeidmann, Manfreden_UK
dc.contributor.authorStrohmeier, Oliveren_UK
dc.contributor.authorvon Stetten, Felixen_UK
dc.contributor.authorDrexler, Josefen_UK
dc.contributor.authorEberhard, Michaelen_UK
dc.contributor.authorNiedrig, Matthiasen_UK
dc.contributor.authorPatel, Pranaven_UK
dc.description.abstractBACKGROUND Yellow fever (YF) is an acute viral hemorrhagic disease transmitted by Aedes mosquitoes. The causative agent, the yellow fever virus (YFV), is found in tropical and subtropical areas of South America and Africa. Although a vaccine is available since the 1930s, YF still causes thousands of deaths and several outbreaks have recently occurred in Africa. Therefore, rapid and reliable diagnostic methods easy to perform in low-resources settings could have a major impact on early detection of outbreaks and implementation of appropriate response strategies such as vaccination and/or vector control. METHODOLOGY The aim of this study was to develop a YFV nucleic acid detection method applicable in outbreak investigations and surveillance studies in low-resource and field settings. The method should be simple, robust, rapid and reliable. Therefore, we adopted an isothermal approach and developed a recombinase polymerase amplification (RPA) assay which can be performed with a small portable instrument and easy-to-use lyophilized reagents. The assay was developed in three different formats (real-time with or without microfluidic semi-automated system and lateral-flow assay) to evaluate their application for different purposes. Analytical specificity and sensitivity were evaluated with a wide panel of viruses and serial dilutions of YFV RNA. Mosquito pools and spiked human plasma samples were also tested for assay validation. Finally, real-time RPA in portable format was tested under field conditions in Senegal. CONCLUSION/SIGNIFICANCE The assay was able to detect 20 different YFV strains and demonstrated no cross-reactions with closely related viruses. The RPA assay proved to be a robust, portable method with a low detection limit (<21 genome equivalent copies per reaction) and rapid processing time (<20 min). Results from real-time RPA field testing were comparable to results obtained in the laboratory, thus confirming our method is suitable for YFV detection in low-resource settings.en_UK
dc.publisherPublic Library of Scienceen_UK
dc.relationEscadafal C, Faye O, Sall AA, Faye O, Weidmann M, Strohmeier O, von Stetten F, Drexler J, Eberhard M, Niedrig M & Patel P (2014) Rapid molecular assays for the detection of yellow Fever virus in low-resource settings. PLoS Neglected Tropical Diseases, 8 (3), Art. No.: e2730.
dc.rights© 2014 Escadafal et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_UK
dc.titleRapid molecular assays for the detection of yellow Fever virus in low-resource settingsen_UK
dc.typeJournal Articleen_UK
dc.citation.jtitlePLoS Neglected Tropical Diseasesen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationRobert Koch Instituteen_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute for Micromachining and Information Technology (HSG-IMIT)en_UK
dc.contributor.affiliationAlbert Ludwigs University of Freiburgen_UK
dc.contributor.affiliationRobert Koch Instituteen_UK
dc.contributor.affiliationRobert Koch Instituteen_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorEscadafal, Camille|en_UK
local.rioxx.authorFaye, Oumar|en_UK
local.rioxx.authorSall, Amadou A|en_UK
local.rioxx.authorFaye, Ousmane|en_UK
local.rioxx.authorWeidmann, Manfred|0000-0002-7063-7491en_UK
local.rioxx.authorStrohmeier, Oliver|en_UK
local.rioxx.authorvon Stetten, Felix|en_UK
local.rioxx.authorDrexler, Josef|en_UK
local.rioxx.authorEberhard, Michael|en_UK
local.rioxx.authorNiedrig, Matthias|en_UK
local.rioxx.authorPatel, Pranav|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameplos neglected tropical diseases 2014.pdfen_UK
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