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dc.contributor.authorNikolay, Birgiten_UK
dc.contributor.authorWeidmann, Manfreden_UK
dc.contributor.authorDupressoir, Anneen_UK
dc.contributor.authorFaye, Ousmaneen_UK
dc.contributor.authorBoye, Cheikh Sen_UK
dc.contributor.authorDiallo, Mawlouthen_UK
dc.contributor.authorSall, Amadou Aen_UK
dc.description.abstractUsutu virus (USUV) has been isolated in several African and European countries mainly from mosquitoes and birds. However, previous benign and two recent severe cases of human infections point out the need of a tool for the identification of USUV in human samples. A published real-time reverse transcription (RT) PCR assay for the detection of USUV in human blood or cerebrospinal fluid does not take into account the genetic variability of USUV in different geographic regions. Therefore, this article presents a quantitative real-time RT-PCR assay based on sequences from Europe and Africa. Primers and probe were designed in conserved regions among USUV strains that differed from closely related flaviviruses. The specificity of the assay was investigated by testing 16 other flaviviruses circulating in Africa. The sensitivity was determined by testing serial dilutions of virus and RNA standard. Intra- and inter-assay coefficients of variation were evaluated by 10 reactions in a same and in different assays, respectively. The assay provides high analytical specificity for USUV and detection limits of 1.2pfu/reaction for virus dilutions in L-15 medium or human serum and 60 copies/reaction for the RNA standard. The assay needs to be evaluated in a clinical context and integrated in standard diagnosis of flaviviral diseases.en_UK
dc.relationNikolay B, Weidmann M, Dupressoir A, Faye O, Boye CS, Diallo M & Sall AA (2014) Development of a Usutu virus specific real-time reverse transcription PCR assay based on sequenced strains from Africa and Europe. Journal of Virological Methods, 197, pp. 51-54.
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.en_UK
dc.subjectUsutu virusen_UK
dc.subjectReal-time RT-PCRen_UK
dc.subjectStandard RNAen_UK
dc.titleDevelopment of a Usutu virus specific real-time reverse transcription PCR assay based on sequenced strains from Africa and Europeen_UK
dc.typeJournal Articleen_UK
dc.citation.jtitleJournal of Virological Methodsen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationUniversity of Viennaen_UK
dc.contributor.affiliationInstitut Pasteuren_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorNikolay, Birgit|en_UK
local.rioxx.authorWeidmann, Manfred|0000-0002-7063-7491en_UK
local.rioxx.authorDupressoir, Anne|en_UK
local.rioxx.authorFaye, Ousmane|en_UK
local.rioxx.authorBoye, Cheikh S|en_UK
local.rioxx.authorDiallo, Mawlouth|en_UK
local.rioxx.authorSall, Amadou A|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameJournal of Virological Methods.pdfen_UK
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