|dc.contributor.author||Tocher, Douglas R||-|
|dc.description.abstract||Biosynthesis in vertebrates of long-chain polyunsaturated fatty acids (LC-PUFA) such as arachidonic (ARA; 20:4n-6), eicosapentaenoic (EPA; 20:5n-3) and docosahexaenoic (DHA; 22:6n-3) acids requires the catalysis by fatty acyl desaturases (Fads). A vertebrate Fad with Δ4 activity catalyzing the direct conversion of 22:5n-3 to DHA was discovered in the marine teleost rabbitfish Siganus canaliculatus. Recent studies in vertebrates have shown that miRNAs may participate in the regulation of lipid metabolism at post-transcription level. However, their roles in LC-PUFA biosynthesis were not known. In the present study, in silico analysis predicts that the rabbitfish Δ4 Fad may be a target of miR-17 and thus we cloned miR-17, which is located at the forepart of the miR-17-92 cluster. Dual luciferase reporter assays demonstrated that miR-17 targeted the 3'UTR of Δ4 Fad directly. Furthermore, the expression level of miR-17 displayed an inverse pattern with that of Δ4 Fad mRNA in gill, liver and eyes, and also the Δ4 Fad protein quantity in rabbitfish liver. Incubation of rabbitfish primary hepatocytes with linoleic acid (LA; 18:2n-6), α-linolenic acid (LNA; 18:3n-3), EPA or DHA showed differential effects on miR-17, Δ4 Fad and Δ6/Δ5 Fad expression. LNA promoted the expression of miR-17 and Δ6/Δ5 Fad, but suppressed the expression of Δ4 Fad. In contrast, LA and EPA decreased the expression of miR-17 and Δ6/Δ5 Fad, but had no effect on Δ4 Fad. However, all the above were down-regulated by DHA. These data indicate that miR-17 was involved in the regulation of LC-PUFA biosynthesis in rabbitfish liver by targeting Δ4 Fad.||en_UK|
|dc.relation||Zhang Q, Xie D, Wang S, You C, Monroig O, Tocher DR & Li Y (2014) miR-17 is involved in the regulation of LC-PUFA biosynthesis in vertebrates: Effects on liver expression of a fatty acyl desaturase in the marine teleost Siganus canaliculatus, Biochimica et Biophysica Acta (BBA)- Molecular and Cell Biology of Lipids, 1841 (7), pp. 934-943.||-|
|dc.rights||Published in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids by Elsevier; Elsevier believes that individual authors should be able to distribute their accepted author manuscripts for their personal voluntary needs and interests, e.g. posting to their websites or their institution’s repository, e-mailing to colleagues. The Elsevier Policy is as follows: Authors retain the right to use the accepted author manuscript for personal use, internal institutional use and for permitted scholarly posting provided that these are not for purposes of commercial use or systematic distribution. An "accepted author manuscript" is the author’s version of the manuscript of an article that has been accepted for publication and which may include any author-incorporated changes suggested through the processes of submission processing, peer review, and editor-author communications.||-|
|dc.title||miR-17 is involved in the regulation of LC-PUFA biosynthesis in vertebrates: Effects on liver expression of a fatty acyl desaturase in the marine teleost Siganus canaliculatus||en_UK|
|dc.citation.jtitle||Biochimica et Biophysica Acta (BBA)- Molecular and Cell Biology of Lipids||-|
|dc.type.status||Post-print (author final draft post-refereeing)||-|
|Appears in Collections:||Aquaculture Journal Articles|
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