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dc.contributor.authorBartie, Kerryen_UK
dc.contributor.authorAustin, Frank Wen_UK
dc.contributor.authorDiab, Ameren_UK
dc.contributor.authorDickson, Cathrynen_UK
dc.contributor.authorDung, Tu Thanhen_UK
dc.contributor.authorGiacomini, Mauroen_UK
dc.contributor.authorCrumlish, Margareten_UK
dc.description.abstractA molecular epidemiology study was conducted on 90 Edwardsiella ictaluri isolates recovered from diseased farmed freshwater catfish, Pangasianodon hypophthalmus, cultured in the Mekong Delta, Vietnam. Thirteen isolates of E. ictaluri derived from diseased channel catfish, Ictalurus punctatus, cultured in the USA were included for comparison. All the E. ictaluri isolates tested were found to be biochemically indistinguishable. A repetitive (rep)-PCR using the single (GTG)5 primer was shown to possess limited discriminatory power, yielding two similar DNA profiles categorized as (GTG)5-PCR group 1 or 2 among the Vietnam isolates and (GTG)5-PCR group 1 within the USA isolates. Macrorestriction analysis identified 14 and 22 unique pulsotypes by XbaI and SpeI, respectively, among a subset of 59 E. ictaluri isolates. Numerical analysis of the combined macrorestriction profiles revealed three main groups: a distinct cluster formed exclusively of the USA isolates, and a major and minor cluster with outliers contained the Vietnam isolates. Antibiotic susceptibility and plasmid profiling supported the existence of the three groups. The results indicate that macrorestriction analysis may be regarded as a suitable typing method among the E. ictaluri species of limited intraspecific diversity. Furthermore, the findings suggest that E. ictaluri originating from Vietnam may constitute a distinct genetic group.en_UK
dc.relationBartie K, Austin FW, Diab A, Dickson C, Dung TT, Giacomini M & Crumlish M (2012) Intraspecific diversity of Edwardsiella ictaluri isolates from diseased freshwater catfish, Pangasianodon hypophthalmus (Sauvage), cultured in the Mekong Delta, Vietnam. Journal of Fish Diseases, 35 (9), pp. 671-682.
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.subjectEdwardsiella ictalurien_UK
dc.subjectPangasianodon hypophthalmusen_UK
dc.subjectpulsed-field gel electrophoresisen_UK
dc.titleIntraspecific diversity of Edwardsiella ictaluri isolates from diseased freshwater catfish, Pangasianodon hypophthalmus (Sauvage), cultured in the Mekong Delta, Vietnamen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[J of FD 2012.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.citation.jtitleJournal of Fish Diseasesen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationMississippi State Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationCollege of Agriculture, Can Thoen_UK
dc.contributor.affiliationUniversity of Genoaen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorBartie, Kerry|0000-0003-3037-5295en_UK
local.rioxx.authorAustin, Frank W|en_UK
local.rioxx.authorDiab, Amer|en_UK
local.rioxx.authorDickson, Cathryn|en_UK
local.rioxx.authorDung, Tu Thanh|en_UK
local.rioxx.authorGiacomini, Mauro|en_UK
local.rioxx.authorCrumlish, Margaret|0000-0002-7810-8172en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameJ of FD 2012.pdfen_UK
Appears in Collections:Aquaculture Journal Articles

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