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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: First identification of Flavobacterium columnare infection in farmed freshwater striped catfish Pangasianodon hypophthalmus
Author(s): Tien, Nguyen Thi
Dung, Tu Thanh
Tuan, Nguyen Ahn
Crumlish, Margaret
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Keywords: Flavobacterium columnare
White patch disease
Pangasianodon hypophthalmus
Issue Date: 13-Aug-2012
Date Deposited: 3-Apr-2014
Citation: Tien NT, Dung TT, Tuan NA & Crumlish M (2012) First identification of Flavobacterium columnare infection in farmed freshwater striped catfish Pangasianodon hypophthalmus. Diseases of Aquatic Organisms, 100 (1), pp. 83-88.
Abstract: The bacterium Flavobacterium columnare was recovered and identified as the aetiological agent causing freshwater columnaris infection in farmed striped catfish Pangasianodon hypophthalmus (Sauvage) fingerlings that had suffered high mortality rates within commercial hatchery ponds in Vietnam. The gross clinical signs were typical of columnaris-infected fish. Histological examination found numerous Gram-negative, filamentous bacteria present on the skin, muscle and gill tissues of affected fish. The yellow-pigmented bacteria were isolated and identified as F. columnare using primary, biochemical and PCR methods. An experimental immersion-challenge study with 2 strains was also performed. It fulfilled Koch's postulates and showed a median lethal concentration (LC50) of 4.27 × 105 and 1.66 × 106 cfu ml-1 for the F. columnare strains FC-HN and FC-CT, respectively. To the best of our knowledge this is the first report of freshwater columnaris infection in P. hypophthalmus.
DOI Link: 10.3354/dao02478
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Publisher policy allows this work to be made available in this repository. Published in Diseases of Aquatic Organisms (2010) Vol 100, No. 1 by Inter-Research. The original publication is available at:

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