Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/19649
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dc.contributor.authorVera, LMen_UK
dc.contributor.authorNegrini, Pietroen_UK
dc.contributor.authorZagatti, Cen_UK
dc.contributor.authorFrigato, Elenaen_UK
dc.contributor.authorSanchez-Vazquez, F Javieren_UK
dc.date.accessioned2015-10-02T00:14:34Z-
dc.date.available2015-10-02T00:14:34Zen_UK
dc.date.issued2013-06en_UK
dc.identifier.urihttp://hdl.handle.net/1893/19649-
dc.description.abstractDaily light and feeding cycles act as powerful synchronizers of circadian rhythmicity. Ultimately, these external cues entrain the expression of clock genes, which generate daily rhythmic behavioral and physiological responses in vertebrates. In the present study, we investigated clock genes in a marine teleost (gilthead sea bream). Partial cDNA sequences of key elements from both positive (Bmal1, Clock) and negative (Per2, Cry1) regulatory loops were cloned before studying how feeding time affects the daily rhythms of locomotor activity and clock gene expression in the central (brain) and peripheral (liver) oscillators. To this end, all fish were kept under a light-dark (LD) cycle and were divided into three experimental groups, depending on the time of their daily meal: mid-light (ML), mid-darkness (MD), or at random (RD) times. Finally, the existence of circadian control on gene expression was investigated in the absence of external cues (DD + RD). The behavioral results showed that seabream fed at ML or RD displayed a diurnal activity pattern (>91% of activity during the day), whereas fish fed at MD were nocturnal (89% of activity during the night). Moreover, seabream subjected to regular feeding cycles (ML and MD groups) showed food-anticipatory activity (FAA). Regardless of the mealtime, the daily rhythm of clock gene expression in the brain peaked close to the light-dark transition in the case of Bmal1 and Clock, and at the beginning of the light phase in the case of Per2 and Cry1, showing the existence of phase delay between the positive and negative elements of the molecular clock. In the liver, however, the acrophases of the daily rhythms differed depending on the feeding regime: the maximum expression of Bmal1 and Clock in the ML and RD groups was in antiphase to the expression pattern observed in the fish fed at MD. Under constant conditions (DD + RD), Per2 and Cry1 showed circadian rhythmicity in the brain, whereas Bmal1, Clock, and Per2 did in the liver. Our results indicate that the seabream clock gene expression is endogenously controlled and in liver it is strongly entrained by food signals, rather than by the LD cycle, and that scheduled feeding can shift the phase of the daily rhythm of clock gene expression in a peripheral organ (liver) without changing the phase of these rhythms in a central oscillator (brain), suggesting uncoupling of the light-entrainable oscillator (LEO) from the food-entrainable oscillator (FEO). These findings provide the basis and new tools for improving our knowledge of the circadian system and entraining pathways of this fish species, which is of great interest for the Mediterranean aquaculture.en_UK
dc.language.isoenen_UK
dc.publisherInforma Healthcareen_UK
dc.relationVera L, Negrini P, Zagatti C, Frigato E & Sanchez-Vazquez FJ (2013) Light and feeding entrainment of the molecular circadian clock in a marine teleost (Sparus aurata). Chronobiology International, 30 (5), pp. 649-661. https://doi.org/10.3109/07420528.2013.775143en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectCentral peripheral oscillatorsen_UK
dc.subjectclock genesen_UK
dc.subjectfeeding entrainmenten_UK
dc.subjectgilthead sea breamen_UK
dc.subjectlocomotor activityen_UK
dc.subjectSparus aurataen_UK
dc.titleLight and feeding entrainment of the molecular circadian clock in a marine teleost (Sparus aurata)en_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31en_UK
dc.rights.embargoreason[Vera et al. CBI 2013.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.3109/07420528.2013.775143en_UK
dc.identifier.pmid23688119en_UK
dc.citation.jtitleChronobiology Internationalen_UK
dc.citation.issn1525-6073en_UK
dc.citation.issn0742-0528en_UK
dc.citation.volume30en_UK
dc.citation.issue5en_UK
dc.citation.spage649en_UK
dc.citation.epage661en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailluisa.veraandujar@stir.ac.uken_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Ferraraen_UK
dc.contributor.affiliationUniversity of Ferraraen_UK
dc.contributor.affiliationUniversity of Ferraraen_UK
dc.contributor.affiliationUniversity of Murciaen_UK
dc.identifier.isiWOS:000320705400002en_UK
dc.identifier.scopusid2-s2.0-84879330971en_UK
dc.identifier.wtid648741en_UK
dc.contributor.orcid0000-0003-0999-055Xen_UK
dc.date.accepted2013-02-07en_UK
dcterms.dateAccepted2013-02-07en_UK
dc.date.filedepositdate2014-03-28en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorVera, LM|0000-0003-0999-055Xen_UK
local.rioxx.authorNegrini, Pietro|en_UK
local.rioxx.authorZagatti, C|en_UK
local.rioxx.authorFrigato, Elena|en_UK
local.rioxx.authorSanchez-Vazquez, F Javier|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2999-12-31en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameVera et al. CBI 2013.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0742-0528en_UK
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