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dc.contributor.authorDel Pozo, Anaen_UK
dc.contributor.authorVera, LMen_UK
dc.contributor.authorSanchez, Jose Aen_UK
dc.contributor.authorSanchez-Vazquez, F Javieren_UK
dc.description.abstractBiological rhythms are driven by circadian oscillators, which are ultimately controlled by the cyclic expression of clock genes. Cryptochromes (CRY), blue light photoreceptors, belong to the negative elements of the transcriptional feedback loop into the molecular clock. This paper describes the cloning and characterization of two cryptochromes (cry1 and 2) in European seabass, which is considered an interesting chronobiology model due to its dual (diurnal/nocturnal) behavior. The cloned cDNA fragments encoded for two proteins of 567 and 668 amino acids, which included the FAD-binding and the DNA-photolyase domains. Moreover, both proteins had a high homology with cryptochrome proteins (Cry) of other teleost fish. These cry1 and 2 genes were expressed in several tissues of seabass (brain, liver, heart, retina, muscle, spleen, gill and intestine). In addition, the daily expression of cry1 was rhythmic in brain, heart and liver with the acrophase around ZT 03:15 h (after the onset of lights). Similarly, the cry2 daily expression was rhythmic in liver, peaking at ZT 03:28 h, whereas in brain the acrophase was at ZT 11:08 h (shortly prior to the offset of lights). These findings provide new elements to help understanding the functioning of the molecular clock of seabass.en_UK
dc.relationDel Pozo A, Vera L, Sanchez JA & Sanchez-Vazquez FJ (2012) Molecular cloning, tissue distribution and daily expression of cry1 and cry2 clock genes in European seabass (Dicentrarchus labrax). Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, 163 (3-4), pp. 364-371.
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.subjectCircadian rhythmen_UK
dc.subjectMolecular clocken_UK
dc.subjectSea bassen_UK
dc.titleMolecular cloning, tissue distribution and daily expression of cry1 and cry2 clock genes in European seabass (Dicentrarchus labrax)en_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[Comp Biochem Physiol A 2012.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.citation.jtitleComparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiologyen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationUniversity of Murciaen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Murciaen_UK
dc.contributor.affiliationUniversity of Murciaen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorDel Pozo, Ana|en_UK
local.rioxx.authorVera, LM|0000-0003-0999-055Xen_UK
local.rioxx.authorSanchez, Jose A|en_UK
local.rioxx.authorSanchez-Vazquez, F Javier|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameComp Biochem Physiol A 2012.pdfen_UK
Appears in Collections:Aquaculture Journal Articles

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