Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/19356
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dc.contributor.authorTeles, Mariana-
dc.contributor.authorBoltana, Sebastian-
dc.contributor.authorReyes-Lopez, Felipe-
dc.contributor.authorSantos, Maria Ana-
dc.contributor.authorMacKenzie, Simon-
dc.contributor.authorTort, Lluis-
dc.date.accessioned2014-03-03T23:29:00Z-
dc.date.issued2013-02-
dc.identifier.urihttp://hdl.handle.net/1893/19356-
dc.description.abstractThe present work was designed to assess the effects of artificially increased high plasma cortisol levels induced by slow-release cortisol implants on the mRNA abundance of the glucocorticoid receptor (GR) in different organs of Sparus aurata (Gilthead sea bream), as well as to evaluate global transcriptional changes in the liver, using the Aquagenomics S. aurata oligo-nucleotide microarray technology. For that purpose, groups of fish were intraperitoneally injected with implants containing two different concentrations of cortisol (50 or 200 μg/g body weight). Blood and organs were sampled after 7 and 14 days of cortisol implantation. Only fish with 200 μg/g implants exhibited a significant rise in plasma cortisol. Thus, we evaluated the expression of the GR in different organs in these fish 7 and 14 days post-implantation. GR mRNA abundance was upregulated in head kidney and heart of fish at both sampling times. In liver and muscle, GR mRNA abundance was upregulated after 14 days, whereas in gills, the GR mRNA transcript was upregulated earlier, at day 7. These results suggest that increased plasma cortisol induced by a slow-release implant of cortisol mimics the overall effects of stress and affects the expression of GR mRNA in a time- and organ-specific manner. Data obtained with the Aquagenomics S. aurata oligo-nucleotide microarray allowed the identification of a total of 491 cortisol-responsive transcripts and highlight the strong intensity of transcriptional modulation in liver of fish implanted with cortisol after 7 days, in contrast to that observed at day 14. Transcriptional remodeling highlighted a significant activity in carbohydrate metabolism mainly in the gluconeogenic pathway linked to downregulation of inflammatory and immune response processes in implanted fish.en_UK
dc.language.isoen-
dc.publisherSpringer-
dc.relationTeles M, Boltana S, Reyes-Lopez F, Santos MA, MacKenzie S & Tort L (2013) Effects of Chronic Cortisol Administration on Global Expression of GR and the Liver Transcriptome in Sparus aurata, Marine Biotechnology, 15 (1), pp. 104-114.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.subjectSparus aurataen_UK
dc.subjectCortisol implantsen_UK
dc.subjectGlucocorticoid receptoren_UK
dc.subjectSAQen_UK
dc.subjectLiveren_UK
dc.titleEffects of Chronic Cortisol Administration on Global Expression of GR and the Liver Transcriptome in Sparus aurataen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31T00:00:00Z-
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.identifier.doihttp://dx.doi.org/10.1007/s10126-012-9467-y-
dc.citation.jtitleMarine Biotechnology-
dc.citation.issn1436-2228-
dc.citation.volume15-
dc.citation.issue1-
dc.citation.spage104-
dc.citation.epage114-
dc.citation.publicationstatusPublished-
dc.citation.peerreviewedRefereed-
dc.type.statusPublisher version (final published refereed version)-
dc.author.emailsebastian.boltana@stir.ac.uk-
dc.contributor.affiliationUniversitat Autonoma de Barcelona-
dc.contributor.affiliationAquaculture-
dc.contributor.affiliationUniversitat Autonoma de Barcelona-
dc.contributor.affiliationUniversity of Aveiro-
dc.contributor.affiliationAquaculture-
dc.contributor.affiliationUniversitat Autonoma de Barcelona-
dc.rights.embargoterms2999-12-31-
dc.rights.embargoliftdate2999-12-31-
dc.identifier.isi000312740300011-
Appears in Collections:Aquaculture Journal Articles

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