Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/18574
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dc.contributor.authorFridman, Sophie-
dc.contributor.authorRana, Kausik-
dc.contributor.authorBron, James-
dc.date.accessioned2015-07-23T05:21:29Z-
dc.date.issued2013-10-
dc.identifier.urihttp://hdl.handle.net/1893/18574-
dc.description.abstractThis study examines the structural differentiation of the apical crypts of mitochondria-rich cells (MRCs) in Nile tilapia as a response to osmotic challenge. Larvae were transferred from freshwater at 3 days post-hatch to 12.5 and 20 ppt and were sampled at 24- and 48-h post-transfer. Scanning electron microscopy allowed quantification of MRCs, based on apical crypt appearance and surface area, resulting in a morphological classification of 'sub-types', that is, Type I or absorptive (surface area range 5.2-19.6 μm(2)), Type II or active absorptive form (surface area range 1.1-15.7 μm(2)), Type III or weakly functioning form (surface area range 0.08-4.6 μm(2)) and Type IV or active secreting form (surface area range 4.1-11.7 μm(2)). Mucus cell crypts were discriminated from those of MRCs based on the presence of globular extensions and quantified. Density and frequency of MRCs and mucus cells varied significantly according to the experimental salinity and time post-transfer; in freshwater-adapted larvae, all types were present except Type IV but, following transfer to elevated salinities, Type I and Type II disappeared and appeared to be replaced by Type IV crypts. Type III crypt density remained constant following transfer. Transmission electron microscopy with immunogold labelling, using a novel pre-fixation technique with anti-Na(+)/K(+)-ATPase, allowed complementary ultrastructural visualisation of specific localisation of the antibodies on active MRCs, permitting a review of MRC apical morphology and related Na(+)/K(+)-ATPase binding sites.en_UK
dc.language.isoen-
dc.publisherSpringer-
dc.relationFridman S, Rana K & Bron J (2013) Structural differentiation of apical openings in active mitochondria-rich cells during early life stages of Nile tilapia (Oreochromis niloticus L.) as a response to osmotic challenge, Fish Physiology and Biochemistry, 39 (5), pp. 1101-1114.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.subjectMitochondria-rich cells Osmoregulationen_UK
dc.subjectCellular differentiationen_UK
dc.subjectNile tilapiaen_UK
dc.subjectOntogenyen_UK
dc.titleStructural differentiation of apical openings in active mitochondria-rich cells during early life stages of Nile tilapia (Oreochromis niloticus L.) as a response to osmotic challengeen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31T00:00:00Z-
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.identifier.doihttp://dx.doi.org/10.1007/s10695-012-9767-1-
dc.identifier.pmid23307174-
dc.citation.jtitleFish Physiology and Biochemistry-
dc.citation.issn0920-1742-
dc.citation.volume39-
dc.citation.issue5-
dc.citation.spage1101-
dc.citation.epage1114-
dc.citation.publicationstatusPublished-
dc.citation.peerreviewedRefereed-
dc.type.statusPublisher version (final published refereed version)-
dc.author.emailj.e.bron@stir.ac.uk-
dc.contributor.affiliationAquaculture-
dc.contributor.affiliationAquaculture-
dc.contributor.affiliationAquaculture-
dc.rights.embargoterms2999-12-31-
dc.rights.embargoliftdate2999-12-31-
dc.identifier.isi000324339500005-
Appears in Collections:Aquaculture Journal Articles

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