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dc.contributor.authorMonaghan, Seanen_UK
dc.contributor.authorThompson, Kimberlyen_UK
dc.contributor.authorAdams, Alexandraen_UK
dc.contributor.authorBergmann, Sven Men_UK
dc.description.abstractKoi herpesvirus (KHV) causes an economically important, highly infectious disease in common carp and koi, Cyprinus carpio L. Since the occurrence of mass mortalities worldwide, highly specific and sensitive molecular diagnostic methods have been developed for KHV detection. The sensitivity and reliability of these assays have essentially focused at the detection of low viral DNA copy numbers during latent or persistent infections. However, the efficacy of these assays has not been investigated with regard to low-level viraemia during acute infection stages. This study was conducted to compare the sensitivity of seven different polymerase chain reaction (PCR) assays to detect KHV during the first hours and days post-infection (hpi; dpi), using lethal and non-lethal sampling methods. The results highlight the limitations of the assays for detecting virus during the first 4 dpi despite rapid mortality in experimentally infected carp. False-negative results were associated with time post-infection and the tissue sampled. Non-lethal sampling appears effective for KHV screening, with efficient detection in mucus samples obtained from external swabs during this early infection period (< 5 dpi), while biopsies from gills and kidney were negative using the same PCR assays. Non-lethal sampling may improve the reliability of KHV detection in subclinical, acutely infected carp.en_UK
dc.relationMonaghan S, Thompson K, Adams A & Bergmann SM (2015) Sensitivity of seven PCRs for early detection of koi herpesvirus in experimentally infected carp, Cyprinus carpio L., by lethal and non-lethal sampling methods [Early KHV detection by 7 PCRs]. Journal of Fish Diseases, 38 (3), pp. 303-319.
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.subjectcyprinid herpesvirus 3en_UK
dc.subjectearly detectionen_UK
dc.subjectKoi herpesvirusen_UK
dc.subjectnon-lethal samplingen_UK
dc.subjectpolymerase chain reactionen_UK
dc.titleSensitivity of seven PCRs for early detection of koi herpesvirus in experimentally infected carp, Cyprinus carpio L., by lethal and non-lethal sampling methodsen_UK
dc.title.alternativeEarly KHV detection by 7 PCRsen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[Monaghan_et_al-2015-Journal_of_Fish_Diseases.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.citation.jtitleJournal of Fish Diseasesen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationFederal Research Institute for Animal Healthen_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorMonaghan, Sean|0000-0002-7692-7756en_UK
local.rioxx.authorThompson, Kimberly|en_UK
local.rioxx.authorAdams, Alexandra|en_UK
local.rioxx.authorBergmann, Sven M|en_UK
local.rioxx.projectInternal Project|University of Stirling|
Appears in Collections:Aquaculture Journal Articles

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