Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/18436
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dc.contributor.authorPourahmad, Fazelen_UK
dc.contributor.authorNemati, Mostafaen_UK
dc.contributor.authorRichards, Randolphen_UK
dc.date.accessioned2014-05-24T23:22:59Z-
dc.date.available2014-05-24T23:22:59Zen_UK
dc.date.issued2014-02en_UK
dc.identifier.urihttp://hdl.handle.net/1893/18436-
dc.description.abstractMycobacterium marinum is a well-recognised mycobacterial species which produces granulomatous reactions in a variety of aquatic organisms. Detection of this organism primarily relies on histological and bacteriological examination of infected fish tissues but recently a number of molecular methods have also been employed. In this study a comparison of three such methods in the detection of M. marinum in goldfish (Carassius auratus) was carried out. Histological analysis revealed that fish infected with M. marinum often developed granulomas in the absence of external lesions and clinical signs of mycobacteriosis. Approximately 40% of all infected fish had greater than/equal to 104 mycobacteria per gram of splenic tissue. Approximately 89% of samples (8/9) with < 104 CFU/g of spleen were positive by PCR. Statistical analysis revealed significant differences between the 3 methods of comparison for the entire sample (N = 15), but very significant differences between PCR and other detection methods (p = 0.0202). Overall, this study demonstrates that PCR is a rapid, specific, and sensitive method for detecting M. marinum in goldfish.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationPourahmad F, Nemati M & Richards R (2014) Comparison of three methods for detection of Mycobacterium marinum in goldfish (Carassius auratus). Aquaculture, 422-423, pp. 42-46. https://doi.org/10.1016/j.aquaculture.2013.11.026en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.subjectDetectionen_UK
dc.subjectMycobacterium marinumen_UK
dc.subjectExperimental infectionen_UK
dc.subjectGoldfishen_UK
dc.titleComparison of three methods for detection of Mycobacterium marinum in goldfish (Carassius auratus)en_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-29en_UK
dc.rights.embargoreason[aquaculture 2014.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1016/j.aquaculture.2013.11.026en_UK
dc.citation.jtitleAquacultureen_UK
dc.citation.issn0044-8486en_UK
dc.citation.volume422-423en_UK
dc.citation.spage42en_UK
dc.citation.epage46en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailr.h.richards@stir.ac.uken_UK
dc.contributor.affiliationIlam Universityen_UK
dc.contributor.affiliationIlam Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000330221900005en_UK
dc.identifier.scopusid2-s2.0-84890280252en_UK
dc.identifier.wtid655105en_UK
dc.date.accepted2013-11-25en_UK
dc.date.filedepositdate2014-01-27en_UK
Appears in Collections:Aquaculture Journal Articles

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