Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/18398
Full metadata record
DC FieldValueLanguage
dc.contributor.authorMota-Velasco, Jose Cen_UK
dc.contributor.authorFerreira, Irani Alvesen_UK
dc.contributor.authorCioffi, Marcelo Ben_UK
dc.contributor.authorOcalewicz, Konraden_UK
dc.contributor.authorCampos-Ramos, Rafaelen_UK
dc.contributor.authorShirak, Andreyen_UK
dc.contributor.authorLee, Bo-Youngen_UK
dc.contributor.authorMartins, Cesaren_UK
dc.contributor.authorPenman, Daviden_UK
dc.date.accessioned2018-04-18T03:58:05Z-
dc.date.available2018-04-18T03:58:05Zen_UK
dc.date.issued2010-07en_UK
dc.identifier.urihttp://hdl.handle.net/1893/18398-
dc.description.abstractOreochromis karongae, one of the "chambo" tilapia species from Lake Malawi, has a karyotype of 2n = 38, making it one of the few species investigated to differ from the typical tilapia karyotype (2n = 44). The O. karongae karyotype consists of one large subtelocentric pair of chromosomes, four medium-sized pairs (three subtelocentric and one submetacentric) and 14 small pairs. The five largest pairs could be distinguished from each other on the basis of size, morphology and a series of fluorescence in situ hybridisation (FISH) probes. The largest pair is easily distinguished on the basis of size and a chromosome 1 (linkage group 3) bacterial artificial chromosome (BAC) FISH probe from Oreochromis niloticus. BAC clones from O. niloticus chromosome 2 (linkage group 7) hybridised to one of the medium-sized subtelocentric chromosome pairs (no. 5) of O. karongae, distinguishing the ancestral medium-sized pair from the three other medium-sized chromosome pairs (nos. 2, 3 and 4) that appear to have resulted from fusions. SATA repetitive DNA hybridised to the centromeres of all 19 chromosome pairs and also revealed the locations of the relic centromeres in the three fused pairs. Telomeric (TTAGGG)n repeats were identified in the telomeres of all chromosomes, and an interstitial telomeric site (ITS) was identified in three chromosomal pairs (no. 2, 3 and 4). Additionally, two ITS sites were identified in the largest chromosome pair (pair 1), confirming the origin of this chromosome from three ancestral chromosomes. SATA and ITS sites allowed the orientation of the fusions in pairs 2, 3 and 4, which all appear to have been in different orientations (q-q, p-q and p-p, respectively). One of these fusions (O. karongae chromosome pair no. 2) involves a small chromosome (equivalent to linkage group 1), which in O. niloticus carries the main sex-determining gene. 4′,6-Diamidino-2-phenyloindole staining of the synaptonemal complex in male O. karongae revealed the presumptive positions of the kinetochores, which correspond well to the centromeric positions observed in the mitotic karyotype.en_UK
dc.language.isoenen_UK
dc.publisherSpringeren_UK
dc.relationMota-Velasco JC, Ferreira IA, Cioffi MB, Ocalewicz K, Campos-Ramos R, Shirak A, Lee B, Martins C & Penman D (2010) Characterisation of the chromosome fusions in Oreochromis karongae. Chromosome Research, 18 (5), pp. 575-586. https://doi.org/10.1007/s10577-010-9141-zen_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjecttilapiaen_UK
dc.subjectchamboen_UK
dc.subjectMalawien_UK
dc.subjectcichliden_UK
dc.subjectFISHen_UK
dc.subjectsynaptonemal complexen_UK
dc.subjectCyp19ben_UK
dc.titleCharacterisation of the chromosome fusions in Oreochromis karongaeen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate3000-01-01en_UK
dc.rights.embargoreason[chromosome research 2010.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1007/s10577-010-9141-zen_UK
dc.identifier.pmid20574823en_UK
dc.citation.jtitleChromosome Researchen_UK
dc.citation.issn1573-6849en_UK
dc.citation.issn0967-3849en_UK
dc.citation.volume18en_UK
dc.citation.issue5en_UK
dc.citation.spage575en_UK
dc.citation.epage586en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emaild.j.penman@stir.ac.uken_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationSao Paulo State University (Universidade Estadual Paulista)en_UK
dc.contributor.affiliationFederal University of Sao Carlosen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationAgricultural Research Organization (ARO)en_UK
dc.contributor.affiliationUniversity of Marylanden_UK
dc.contributor.affiliationSao Paulo State University (Universidade Estadual Paulista)en_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000280125500005en_UK
dc.identifier.scopusid2-s2.0-77956912826en_UK
dc.identifier.wtid886737en_UK
dc.contributor.orcid0000-0001-8608-6631en_UK
dcterms.dateAccepted2010-07-31en_UK
dc.date.filedepositdate2014-01-22en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorMota-Velasco, Jose C|en_UK
local.rioxx.authorFerreira, Irani Alves|en_UK
local.rioxx.authorCioffi, Marcelo B|en_UK
local.rioxx.authorOcalewicz, Konrad|en_UK
local.rioxx.authorCampos-Ramos, Rafael|en_UK
local.rioxx.authorShirak, Andrey|en_UK
local.rioxx.authorLee, Bo-Young|en_UK
local.rioxx.authorMartins, Cesar|en_UK
local.rioxx.authorPenman, David|0000-0001-8608-6631en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate3000-01-01en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenamechromosome research 2010.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0967-3849en_UK
Appears in Collections:Aquaculture Journal Articles

Files in This Item:
File Description SizeFormat 
chromosome research 2010.pdfFulltext - Published Version503.52 kBAdobe PDFUnder Embargo until 3000-01-01    Request a copy


This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved https://creativecommons.org/publicdomain/zero/1.0/

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.