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dc.contributor.authorGuyon, Richarden_UK
dc.contributor.authorRakotomanga, Michaelleen_UK
dc.contributor.authorAzzouzi, Naoualen_UK
dc.contributor.authorCoutanceau, Jean-Pierreen_UK
dc.contributor.authorBonillo, Celineen_UK
dc.contributor.authorD’Cotta, Helenaen_UK
dc.contributor.authorPepey, Elodieen_UK
dc.contributor.authorSoler, Lucileen_UK
dc.contributor.authorRodier-Goud, Margueriteen_UK
dc.contributor.authorD’Hont, Angeliqueen_UK
dc.contributor.authorConte, Matthew Aen_UK
dc.contributor.authorvan Bers, Nikkie E Men_UK
dc.contributor.authorPenman, Daviden_UK
dc.contributor.authorHitte, Christopheen_UK
dc.contributor.authorCrooijmans, Richard P M Aen_UK
dc.description.abstractBackground: The Nile tilapia (Oreochromis niloticus) is the second most farmed fish species worldwide. It is also an important model for studies of fish physiology, particularly because of its broad tolerance to an array of environments. It is a good model to study evolutionary mechanisms in vertebrates, because of its close relationship to haplochromine cichlids, which have undergone rapid speciation in East Africa. The existing genomic resources for Nile tilapia include a genetic map, BAC end sequences and ESTs, but comparative genome analysis and maps of quantitative trait loci (QTL) are still limited. Results: We have constructed a high-resolution radiation hybrid (RH) panel for the Nile tilapia and genotyped 1358 markers consisting of 850 genes, 82 markers corresponding to BAC end sequences, 154 microsatellites and 272 single nucleotide polymorphisms (SNPs). From these, 1296 markers could be associated in 81 RH groups, while 62 were not linked. The total size of the RH map is 34,084 cR3500 and 937,310 kb. It covers 88% of the entire genome with an estimated inter-marker distance of 742 Kb. Mapping of microsatellites enabled integration to the genetic map. We have merged LG8 and LG24 into a single linkage group, and confirmed that LG16-LG21 are also merged. The orientation and association of RH groups to each chromosome and LG was confirmed by chromosomal in situ hybridizations (FISH) of 55 BACs. Fifty RH groups were localized on the 22 chromosomes while 31 remained small orphan groups. Synteny relationships were determined between Nile tilapia, stickleback, medaka and pufferfish. Conclusion:The RH map and associated FISH map provide a valuable gene-ordered resource for gene mapping and QTL studies. All genetic linkage groups with their corresponding RH groups now have a corresponding chromosome which can be identified in the karyotype. Placement of conserved segments indicated that multiple inter-chromosomal rearrangements have occurred between Nile tilapia and the other model fishes. These maps represent a valuable resource for organizing the forthcoming genome sequence of Nile tilapia, and provide a foundation for evolutionary studies of East African cichlid fishes.en_UK
dc.publisherBioMed Centralen_UK
dc.relationGuyon R, Rakotomanga M, Azzouzi N, Coutanceau J, Bonillo C, D’Cotta H, Pepey E, Soler L, Rodier-Goud M, D’Hont A, Conte MA, van Bers NEM, Penman D, Hitte C & Crooijmans RPMA (2012) A high-resolution map of the Nile tilapia genome: a resource for studying cichlids and other percomorphs. BMC Genomics, 13 (1), Art. No.: 222.
dc.rights© 2012 Guyon et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_UK
dc.titleA high-resolution map of the Nile tilapia genome: a resource for studying cichlids and other percomorphsen_UK
dc.typeJournal Articleen_UK
dc.citation.jtitleBMC Genomicsen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.description.notesAdditional co-authors: Thomas D Kocher, Catherine Ozouf-Costaz, Jean Francois Baroiller and Francis Galiberten_UK
dc.contributor.affiliationUniversity of Rennesen_UK
dc.contributor.affiliationUniversity of Rennesen_UK
dc.contributor.affiliationUniversity of Rennesen_UK
dc.contributor.affiliationMuseum National d’Histoire Naturelleen_UK
dc.contributor.affiliationUniversity of Marylanden_UK
dc.contributor.affiliationWageningen Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Rennesen_UK
dc.contributor.affiliationWageningen Universityen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorGuyon, Richard|en_UK
local.rioxx.authorRakotomanga, Michaelle|en_UK
local.rioxx.authorAzzouzi, Naoual|en_UK
local.rioxx.authorCoutanceau, Jean-Pierre|en_UK
local.rioxx.authorBonillo, Celine|en_UK
local.rioxx.authorD’Cotta, Helena|en_UK
local.rioxx.authorPepey, Elodie|en_UK
local.rioxx.authorSoler, Lucile|en_UK
local.rioxx.authorRodier-Goud, Marguerite|en_UK
local.rioxx.authorD’Hont, Angelique|en_UK
local.rioxx.authorConte, Matthew A|en_UK
local.rioxx.authorvan Bers, Nikkie E M|en_UK
local.rioxx.authorPenman, David|0000-0001-8608-6631en_UK
local.rioxx.authorHitte, Christophe|en_UK
local.rioxx.authorCrooijmans, Richard P M A|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameBMC Genomics 2012.pdfen_UK
Appears in Collections:Aquaculture Journal Articles

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