Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/18312
Full metadata record
DC FieldValueLanguage
dc.contributor.authorEuler, Milena-
dc.contributor.authorWang, Yongjie-
dc.contributor.authorNentwich, Oliver-
dc.contributor.authorPiepenburg, Olaf-
dc.contributor.authorHufert, Frank T-
dc.contributor.authorWeidmann, Manfred-
dc.date.accessioned2014-01-14T23:44:18Z-
dc.date.issued2012-08-
dc.identifier.urihttp://hdl.handle.net/1893/18312-
dc.description.abstractBackground: Detection of nucleic acids of Rift Valley fever virus (RVFV) has been shown to be useful in field diagnostics. Objectives: To develop an isothermal ‘recombinase polymerase amplification (RPA)' assay on an ESEquant tubescanner device. Study design: RPA was adapted for RNA amplification by first developing a two-step and then a one-step-RT-RPA protocol. Several RT enzymes were tested and the best sensitivity was achieved using Transcriptor (Roche). Finally an RT-RPA pellet containing a recombinant MuLV was tested in RVFV one-step-RT-RPA. Results: The one-step-RT-RPA assay showed a sensitivity of 19 molecules detected as determined by probit analysis of eight runs using a RVFV S-segment based quantitative RNA standard and detected 20 different RVFV strains. The assays showed no cross detection of the human genome and several agents of a typical biothreat panel. It performed almost as good as the assay using glycerol buffer based Transcriptor albeit at a cost of 1-log10 step in sensitivity. The presented combination of one-step-RT-RPA and portable fluorescence reading device could be a useful tool for field or point of care diagnostics.en_UK
dc.language.isoen-
dc.publisherElsevier-
dc.relationEuler M, Wang Y, Nentwich O, Piepenburg O, Hufert FT & Weidmann M (2012) Recombinase polymerase amplification assay for rapid detection of Rift Valley fever virus, Journal of Clinical Virology, 54 (4), pp. 308-312.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.subjectRPAen_UK
dc.subjectRecombinase polymerase amplificationen_UK
dc.subjectRift Valley fever virusen_UK
dc.titleRecombinase polymerase amplification assay for rapid detection of Rift Valley fever virusen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31T00:00:00Z-
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.identifier.doihttp://dx.doi.org/10.1016/j.jcv.2012.05.006-
dc.citation.jtitleJournal of Clinical Virology-
dc.citation.issn1386-6532-
dc.citation.volume54-
dc.citation.issue4-
dc.citation.spage308-
dc.citation.epage312-
dc.citation.publicationstatusPublished-
dc.citation.peerreviewedRefereed-
dc.type.statusPublisher version (final published refereed version)-
dc.author.emailm.w.weidmann@stir.ac.uk-
dc.contributor.affiliationUniversity of Gottingen, Georg-August University-
dc.contributor.affiliationUniversity of Gottingen, Georg-August University-
dc.contributor.affiliationTwistDx-
dc.contributor.affiliationTwistDx-
dc.contributor.affiliationUniversity of Gottingen, Georg-August University-
dc.contributor.affiliationAquaculture-
dc.rights.embargoterms2999-12-31-
dc.rights.embargoliftdate2999-12-31-
dc.identifier.isi000306374700004-
Appears in Collections:Aquaculture Journal Articles

Files in This Item:
File Description SizeFormat 
Journal of Clinical Virology 2012.pdf290.2 kBAdobe PDFUnder Permanent Embargo    Request a copy


This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.